Comprehensive Analyses of Quercetin Conjugates by LC/MS/MS Revealed That Isorhamnetin-7-O-glucuronide-4′-O-sulfate Is a Major Metabolite in Plasma of Rats Fed with Quercetin Glucosides
Comprehensive Analyses of Quercetin Conjugates by LC/MS/MS Revealed That Isorhamnetin-7-O-glucuronide-4′-O-sulfate Is a Major Metabolite in Plasma of Rats Fed with Quercetin Glucosides
复制标题
通过 LC/MS/MS 对槲皮素缀合物进行综合分析表明，异鼠李素-7-O-葡萄糖醛酸-4-O-硫酸盐是喂食槲皮素葡萄糖苷的大鼠血浆中的主要代谢物
DOI：
10.1021/acs.jafc.8b06929
复制
发表时间：
2019
影响因子：
6.1
通讯作者：
Hara Hiroshi
中科院分区：
文献类型：
--
作者：
Tanaka Seiya;Trakooncharoenvit Aphichat;Nishikawa Miyu;Ikushiro Shinichi;Hara Hiroshi
饮食中的槲皮素糖苷被吸收，并在血浆中转化为葡萄糖醛酸苷、硫酸盐或葡萄糖醛酸苷和硫酸盐的混合结合物。栎素结合物(Q-结合物)的生理作用因结合类型和位置的不同而不同。用LC/MS/MS对包括混合偶联物在内的Q-偶联物进行了全面的分析。在饲喂含0.24%槲皮苷的饲料的大鼠中，分别在第1、3、12天测定了尾血中Q-偶联物的种类。检测到23个Q-共轭分子，其中16个Q-共轭分子用标准化合物进行了定量。血浆中最丰富的代谢物是异鼠李素-7-O-葡萄糖醛酸苷-4‘-O-硫酸盐，其次是槲皮素-7-O-葡萄糖醛酸-4’-O-硫酸盐，合计占第12天总Q-结合物的86%。用我们的方法定量的总的Q-共轭分子与用酶去共轭方法定量的总的Q-共轭分子是相似的。
Quercetin glycosides in the diet are absorbed and converted to glucuronides, sulfates, or mixed conjugates of glucuronide and sulfate in plasma. Physiological effects of quercetin conjugates (Q-conjugates) differ depending on the type and position of conjugation. We developed a comprehensive analysis of Q-conjugates, including mixed conjugates, by LC/MS/MS. The whole species of Q-conjugates in tail blood plasma was measured on days 1, 3, and 12 in rats fed a 0.24% quercetin glucoside-containing diet. Twenty-three Q-conjugate molecules were detected, and 16 Q-conjugates among these were quantified using standard compounds. The most abundant metabolite in the plasma was mixed conjugates, comprising isorhamnetin-7-O-glucuronide-4′-O-sulfate, followed by quercetin-7-O-glucuronide-4′-O-sulfate; together, they accounted for 86% of total Q-conjugates on day 12. The profile of quercetin conjugate species did not significantly change during 12 days. The total Q-conjugate molecules quantified by our method was comparable with the total Q-conjugates quantified using an enzymatic deconjugation method.