Corneal abnormalities in the NC/Nga mouse - An atopic dermatitis model
Corneal abnormalities in the NC/Nga mouse - An atopic dermatitis model
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DOI：
10.1097/ico.0b013e31816f6328
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发表时间：
2008-09-01
期刊：
影响因子：
2.8
通讯作者：
Ra, Chisei
中科院分区：
文献类型：
--
作者：
Ebihara, Nobuyuki;Funaki, Toshinari;Ra, Chisei
目的：研究异位性皮炎动物模型NC/Nga小鼠角膜异常。方法：对眼睑、结膜、角膜的组织病理学变化进行研究，取眼球及上下眼睑固定，光镜、电镜检查或快速冷冻免疫组化。采用转移酶介导的dutp -地高辛镍端标记法检测凋亡细胞。为了评估眼抓行为和水合他克柳铵软膏的效果，我们进行了录像。结果：常规房间小鼠出现不同程度的眼睑结膜炎，并剧烈抓眼。水合他克莫司软膏减少了它们的抓眼行为。眼睑和结膜的组织病理学检查表明，眼睑结膜炎是由过敏性炎症引起的。严重眼睑结膜炎小鼠表现为角膜上皮变薄，上皮与间质间界面不规则，上皮下物质沉积，间质新生血管。他们的角膜呈锥形。在浅表上皮细胞和角质细胞中发现了许多转移酶介导的dutp -地高辛镍端标记阳性细胞。结论：NC/Nga小鼠是一种有效的特应性眼睑结膜炎动物模型。这些小鼠的各种角膜疾病可能与它们的抓眼行为有关。
Purpose: To Study corneal abnormalities in the NC/Nga Mouse, which is an animal model of atopic dermatitis.Methods: To study histopathologic changes of the eyelid, conjunctiva, and cornea, we extracted the eyeballs together with upper and lower eyelids and fixed them for examination by light and electron microscopy or snap-froze them for immunohistochemistry. Transferase mediated-dUTP digoxigenin nick-end labeling staining was performed to detect apoptotic cells. In order to assess eye scratching behavior and the effect of tacroliums hydrate ointment, we made video recordings.Results: Mice kept in a conventional room suffered from various grades of blepharoconjunctivitis and scratched their eyes furiously. Tacrolimus hydrate ointment reduced their eye-scratching behavior. Histopathologic study of the eyelid and conjunctiva showed that this blepharoconjunctivitis was caused by allergic inflammation. Mice with severe blepharoconjunctivitis showed thinning of the corneal epithelium, an irregular interface between the epithelium and stroma, subepithelial deposition of materials, and neovascularization of the stroma. Their corneas were cone shaped. Many transferase mediated-dUTP digoxigenin nick-end labeling-positive cells were recognized among superficial epithelial cells and keratocytes.Conclusions: NC/Nga mice are a useful animal model of atopic blepharoconjunctivitis. Various corneal disorders in these mice may depend on their eye-scratching behavior.