Luciferase Complementation Assay for Protein-Protein Interactions in Plants
Luciferase Complementation Assay for Protein-Protein Interactions in Plants
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DOI：
10.1002/cppb.20066
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Jian‐Min Zhou
中科院分区：
文献类型：
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作者：
Zhaoyang Zhou;Guozhi Bi;Jian‐Min Zhou
Constitutive and dynamic protein-protein interactions are fundamental to all.aspects of cellular processes. Compared to other techniques measuring proteinprotein.interactions in plants, the luciferase complementation assay has a number.of advantages: it detects plant protein-protein interactions in real time,.requires little hands-on manipulation of samples, is highly quantitative, has.extremely low background, and can be easily scaled up for high-throughput interactome.studies. Here, we describe a protocol that includes two alternate data.collection methods to quantify luminescence results based on Agrobacteriummediated.transient luciferase expression in Nicotiana benthamiana. One data.collection method employs a charge-coupled device imaging system that allows.the interactions to be presented as images, and the other employs a luminometer,.which enables the assay to be conducted in a 96-well plate. Technical.parameters related to frequently encountered problems and common errors,.presented here, are important for performing this assay successfully.
