Basis for the phototaxis sign reversal in the green alga Chlamydomonas reinhardtii studied by high-speed observation
Basis for the phototaxis sign reversal in the green alga Chlamydomonas reinhardtii studied by high-speed observation
复制标题
高速观测研究绿藻莱茵衣藻趋光性信号反转的基础
DOI：
10.1101/2020.12.06.414052
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Wakabayashi Ken-ichi
中科院分区：
文献类型：
--
作者：
Nakajima Masako;Iizuka Kosuke;Ueki Noriko;Isu Atsuko;Yoshimura Kenjiro;Nakagaki Toshiyuki;Hisabori Toru;Sato Katsuhiko;Wakabayashi Ken-ichi
对于利用税收对环境刺激做出反应的生物体，为了生存，应该严格控制策略标志的反转。双纤毛绿藻--衣藻衣藻是研究正、负趋光性逆转的极佳模型。雷氏衣藻细胞在眼点受光后，通过调节两纤毛之间的打击力平衡来改变游动方向；然而，它们如何逆转趋光性符号仍不清楚。在这项研究中，我们用高速摄像机观察细胞的趋光性转弯，发现两个关键因素决定了趋光性标志：两种纤毛中的哪一种在趋光性转弯中跳动得更强烈，以及何时开始强烈跳动。强烈纤毛跳动的时机被认为是由ROS调节的眼点点亮和点亮反应之间的切换来调节的，这导致了正趋光性和负趋光性之间的切换。这一观点得到了一个数学模型的支持，该模型引入了光接收后强烈纤毛跳动的时间。
For organisms that respond to environmental stimuli using taxes, reversal of the tactic sign should be tightly regulated for survival. The biciliate green algaChlamydomonas reinhardtiiis an excellent model for studying reversal between positive and negative phototaxis.C. reinhardtiicells change swimming direction by modulating the balance of beating forces between their two cilia after photoreception at the eyespot; however, it remains unknown how they reverse phototactic sign. In this study, we observed cells undergoing phototactic turns with a high-speed camera and found that two key factors determine the phototactic sign: which of the two cilia beats more strongly for phototactic turning and when the strong beating starts. The timing of the strong ciliary beating is suggested to be regulated by ROS-regulated switching between the light-on and light-off responses at the eyespot, which leads to the switching between positive and negative phototaxis. This idea is supported by a mathematical model that introduces the timing of the strong ciliary beating after photoreception.
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影响因子：
--
作者：
RUFFER, U;NULTSCH, W
通讯作者：
NULTSCH, W
光合作用调节野生型莱茵衣藻的趋光性迹象
影响因子：
3.5
作者：
Tetsuo Takahashi;Masakatsu Watanabe
通讯作者：
Masakatsu Watanabe
莱茵衣藻两条鞭毛之间节拍频率的内在差异。
影响因子：
3.7
作者：
Ritsu Kamiya;Etsuko Hasegawa
通讯作者：
Etsuko Hasegawa
影响因子：
6.4
作者：
FEINLEIB, ME;CURRY, GM
通讯作者：
CURRY, GM
通过氧化还原平衡调节莱氏衣藻鞭毛运动。
DOI：
--
发表时间：
2006
期刊：
The Journal of Cell Biology 173(5)
影响因子：
--
作者：
Wakabayashi;K;King;S. M.
通讯作者：
S. M.