THE EFFECT OF APPARATUS, EXTRACTION TIME, AND SOLVENT TYPE ON LIPID EXTRACTIONS OF SNOW GEESE
THE EFFECT OF APPARATUS, EXTRACTION TIME, AND SOLVENT TYPE ON LIPID EXTRACTIONS OF SNOW GEESE
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DOI：
10.1139/z85-285
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发表时间：
1985-01-01
影响因子：
1.4
通讯作者：
KREMENTZ, DG
中科院分区：
文献类型：
--
作者：
DOBUSH, GR;ANKNEY, CD;KREMENTZ, DG
在文献中，脂质提取的仪器、溶剂类型和提取时间的差异是常见的。为了研究这种方法学上的差异对所提取物质的量和类型的影响，将雪雁（Chen caerulescens）匀浆的等量子样本分别在索氏提取器和戈德菲施脂肪提取器中，用石油醚、乙醚、氯仿 - 甲醇以及石油醚 - 氯仿 - 甲醇混合物提取3、6、12和24小时。所使用的溶剂类型对提取物质的量影响最大。石油醚和乙醚提取的非脂质很少，但氯仿 - 甲醇提取了相对大量的非脂质。我们得出结论，当在戈德菲施或索氏脂肪提取器中使用时，氯仿 - 甲醇对于涉及身体成分的研究是一种不合适的溶剂。
Variability in apparatus, solvent type, and extraction time for lipid extractions is common in the literature. To investigate the effect of this methodological variability on the amount and type of material extracted, equal subsamples of snow goose (Chen caerulescens) homogenate were extracted in Soxhlet and Goldfisch fat extractors with petroleum ether, diethyl ether, chloroform-methanol, and a petroleum ether-chloroform-methanol mixture for 3, 6, 12, and 24 h. The type of solvent used had the largest effect on the amount of material that was extracted. Petroleum ether and diethyl ether extracted few nonlipids, but chloroform-methanol extracted relatively large amounts of nonlipds. We conclude that chloroform-methanol, when used in a Goldfisch or Soxhlet fat extractor, is an inappropriate solvent for studies dealing with body composition.