Crystallization and preliminary X-ray analysis of aspartate racemase from Pyrococcus horikoshii OT3
Crystallization and preliminary X-ray analysis of aspartate racemase from Pyrococcus horikoshii OT3
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DOI：
10.1107/s0907444901012549
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发表时间：
2001-11-01
影响因子：
2.2
通讯作者：
Miki, K
中科院分区：
文献类型：
--
作者：
Liu, LJ;Iwata, K;Miki, K
本文报道了用坐滴气相扩散法从Pyrococcus horikoshii OT 3（P.AspR）中分离出的天冬氨酸消旋酶的三种晶型。晶体属于空间群P2（1），P2（1）2（1）2（1）和P3（1）2（1）（或P3（2）21）。空间群为P2（1）的晶体在90 K液氮低温条件下用同步辐射可吸收1.7埃分辨率以上的X射线，并被选择用于结构测定。用浸渍法得到了该晶体的两个重原子衍生物，并给出了初始电子密度图。
Aspartate racemase from Pyrococcus horikoshii OT3 (P. AspR) has been crystallized in three crystal forms by the sitting-drop vapour-diffusion method. The crystals belong to the space groups P2(1), P2(1)2(1)2(1) and P3(1)2(1) (or P3(2)21). The crystals of space group P2(1) diffract X-rays beyond 1.7 Angstrom resolution under 90 K liquid-nitrogen cryo-conditions with synchrotron radiation and were selected for structure determination. Two heavy-atom derivatives of this crystal form were obtained by the soaking method, which afforded the initial electron-density map.