Light-controlled scaffold- and serum-free hard palatal-derived mesenchymal stem cell aggregates for bone regeneration.
Light-controlled scaffold- and serum-free hard palatal-derived mesenchymal stem cell aggregates for bone regeneration.
复制标题
DOI：
10.1002/btm2.10334
复制
发表时间：
2023-01
影响因子：
7.4
通讯作者：
中科院分区：
文献类型：
--
作者：
模拟体内细胞-细胞相互作用的细胞聚集体是组织工程的有前途和强大的工具。本研究从大鼠硬腭中分离出一种新的、易于获得的间充质干细胞（MSCs）群体，命名为硬腭源性间充质干细胞（PMSCs）。PMSC对CD 90、CD 44和CD 29呈阳性，对CD 34、CD 45和CD 146呈阴性。它们表现出克隆形成、自我更新、迁移和多能分化能力。此外，本研究使用光控细胞片技术和无血清方法制备无支架3D聚集体。成功构建了具有良好生存能力的PMSC聚集体。PMSC聚集体和PMSC聚集体-种植体复合物的移植分别显著增强了体内骨形成和种植体骨整合。这种新的细胞资源易于获得，并为组织工程和再生医学提供了一种替代策略。
Cell aggregates that mimic in vivo cell–cell interactions are promising and powerful tools for tissue engineering. This study isolated a new, easily obtained, population of mesenchymal stem cells (MSCs) from rat hard palates named hard palatal‐derived mesenchymal stem cells (PMSCs). The PMSCs were positive for CD90, CD44, and CD29 and negative for CD34, CD45, and CD146. They exhibited clonogenicity, self‐renewal, migration, and multipotent differentiation capacities. Furthermore, this study fabricated scaffold‐free 3D aggregates using light‐controlled cell sheet technology and a serum‐free method. PMSC aggregates were successfully constructed with good viability. Transplantation of the PMSC aggregates and the PMSC aggregate‐implant complexes significantly enhanced bone formation and implant osseointegration in vivo, respectively. This new cell resource is easy to obtain and provides an alternative strategy for tissue engineering and regenerative medicine.