Non-invasive evaluation of atopic dermatitis based on redox status using in vivo dynamic nuclear polarization magnetic resonance imaging
Non-invasive evaluation of atopic dermatitis based on redox status using in vivo dynamic nuclear polarization magnetic resonance imaging
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DOI：
10.1016/j.freeradbiomed.2016.12.043
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发表时间：
2017-02-01
影响因子：
7.4
通讯作者：
Hyodo, Fuminori
中科院分区：
文献类型：
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作者：
Eto, Hinako;Tsuji, Gaku;Hyodo, Fuminori
特应性皮炎（AD）是一种慢性炎症性疾病，病因复杂，包括遗传、环境和免疫因素。过度氧化应激引起的氧化还原失衡已被证明可以介导AD的疾病活动。目前，尚未开发出能够监测体内皮肤氧化还原状态的成像技术。因此，我们建立了一种可以使用体内动态核极化磁共振成像（DNP-MRI）检测和可视化皮肤氧化还原状态的技术。为了评估这项技术，我们利用了 AD 小鼠模型，该模型是通过在 NC/Nga 小鼠中重复局部应用螨抗原而产生的。我们对小鼠 AD 皮肤损伤的氧化还原平衡变化进行了成像。使用体内 DNP-MRI 和无毒硝酰自由基来可视化体内自由基，我们发现 AD 皮肤病变表现出比正常皮肤更快的图像强度增强衰减率，表明我们的技术可以监测 AD 皮肤病变中发生的过度氧化应激。因此，该技术有可能从改变氧化还原状态的角度提供一种评估炎症性皮肤病（包括 AD）疾病活动性的新方法。
Atopic dermatitis (AD) is a chronic inflammatory condition with complex etiology, including genetic, environmental and immunologic factors. Redox imbalance caused by excessive oxidative stress has been shown to mediate disease activity of AD. Currently, an imaging technique that can monitor the redox status of the skin in vivo has not yet been developed. Consequently, we have established such a technique that can detect and visualize the redox status of the skin using in vivo dynamic nuclear polarization magnetic resonance imaging (DNP-MRI). To evaluate this technique, we utilized an AD mouse model that was generated by repeated topical application of mite antigen in NC/Nga mice. We imaged alterations in redox balance of the resulting AD skin lesions of the mice. Using in vivo DNP-MRI and non-toxic nitroxyl radicals to visualize free radicals in vivo, we revealed that AD skin lesions demonstrated more rapid decay rates of image intensity enhancement than normal skin, indicating that our technique can monitor excessive oxidative stress occurring in AD skin lesions. Therefore, this technique has the potential to provide a novel approach for evaluating disease activity of inflammatory skin diseases, including AD, from the view point of altered redox status.