Stimulation of epithelial cell matrix metalloproteinase (MMP-2,-9,-13) and interleukin-8 secretion by fusobacteria
Stimulation of epithelial cell matrix metalloproteinase (MMP-2,-9,-13) and interleukin-8 secretion by fusobacteria
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DOI：
10.1111/j.1399-302x.2008.00453.x
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发表时间：
2008-10-01
影响因子：
--
通讯作者：
Uitto, V. -J.
中科院分区：
文献类型：
--
作者：
Gursoy, U. K.;Koenonen, E.;Uitto, V. -J.
背景/目的：参与牙周病的细菌主要通过刺激宿主细胞分泌促炎细胞因子和基质金属蛋白酶(MMPs)来发挥其破坏性作用。本研究旨在检测梭菌对上皮细胞基质金属蛋白酶和白介素8(IL-8)分泌的影响。方法：将8株不同的口腔和非口腔梭菌与HaCaT上皮细胞共同培养。结果：所有梭杆菌，尤其是坏死梭杆菌ATCC 25286、核梭杆菌ATCC 25586和变种梭杆菌ATCC 51644均能促进上皮细胞分泌胶原酶3、明胶酶A、明胶酶B和分泌IL-8。模拟梭杆菌ATCC 33568、核梭杆菌和坏死梭菌均能促进上皮细胞分泌基质金属蛋白酶-2。核假单胞菌和坏血假单胞菌也能促进IL-8的分泌。变种镰刀菌ATCC 27725只弱刺激基质金属蛋白酶的产生，强烈促进IL-8的产生，提示它们的表达受到不同的调控。结论：梭菌的致病潜能可能部分源于它们刺激上皮细胞分泌基质金属蛋白酶9、13和IL-8的能力。
Background/aim: Bacterial pathogens involved in periodontal diseases exert their destructive effects primarily by stimulating the host cells to increase their secretion of proinflammatory cytokines and matrix metalloproteinases (MMPs). This study aimed to determine the epithelial cell matrix metalloproteinase and interleukin-8 (IL-8) secretion upon exposure to fusobacteria.Methods: Eight different oral and non-oral Fusobacterium strains were incubated with HaCaT epithelial cells. Gelatin zymography and Western blot analysis were performed to detect collagenase 3 (MMP-13), gelatinase A (MMP-2), gelatinase B (MMP-9), and IL-8 secretion by epithelial cells.Results: All Fusobacterium strains, especially Fusobacterium necrophorum ATCC 25286, Fusobacterium nucleatum ATCC 25586, and Fusobacterium varium ATCC 51644, increased MMP-9 and MMP-13 secretion. Fusobacterium simiae ATCC 33568, and to a lesser extent F. nucleatum and F. necrophorum, increased epithelial MMP-2 secretion. F. nucleatum and F. necrophorum also increased IL-8 secretion. F. varium ATCC 27725, a strain that only weakly stimulated MMP production, strongly increased the IL-8 production, suggesting that their expression is differently regulated.Conclusion: We conclude that the pathogenic potential of fusobacteria may partly result from their ability to stimulate secretion of MMP-9, MMP-13, and IL-8 from epithelial cells.