Mitochondria Na(+)-Ca (2+) exchange in cardiomyocytes and lymphocytes.
Mitochondria Na(+)-Ca (2+) exchange in cardiomyocytes and lymphocytes.
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DOI：
10.1007/978-1-4614-4756-6_16
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发表时间：
2013
影响因子：
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通讯作者：
Bongju Kim;A. Takeuchi;Orie Koga;M. Hikida;S. Matsuoka
中科院分区：
文献类型：
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作者：
Bongju Kim;A. Takeuchi;Orie Koga;M. Hikida;S. Matsuoka
线粒体 Na+-Ca2+ 交换 (NCXmit) 最初由 Carafoli 等人发现。 1974年。此后，NCXmit的机制和作用得到了广泛的研究。我们通过介绍我们最近的研究来回顾 NCXmitin 心肌细胞和淋巴细胞。对 NCXmitin 大鼠心室细胞的研究表明，NCXmitin 具有电压依赖性和产电性。分别在 HL-1 心肌细胞和 B 淋巴细胞中靶向敲低和敲除 NCLX，显着降低了 NCXmit 活性，表明 NCLX 是这些细胞中 NCXmitin 的主要成分。在 NCLX 敲除淋巴细胞中，钙池操纵的 Ca2+ 进入大大减弱，表明大量 Ca2+ 进入线粒体并通过 NCXmit 释放到细胞质中。 NCXmitor NCLX 在线粒体和细胞质中的 Ca2+ 处理中具有关键作用。
Mitochondria Na+-Ca2+exchange (NCXmit) was first discovered by Carafoli et al. in 1974. Thereafter, the mechanisms and roles of NCXmithave been extensively studied. We review NCXmitin cardiomyocytes and lymphocytes by presenting our recent studies on it. Studies of NCXmitin rat ventricular cells demonstrated that NCXmitis voltage dependent and electrogenic. A targeted knockdown and knockout of NCLX in HL-1 cardiomyocytes and B lymphocytes, respectively, significantly reduced the NCXmitactivity, indicating that NCLX is a major component of NCXmitin these cells. The store-operated Ca2+entry was greatly attenuated in NCLX knockout lymphocytes, suggesting that substantial amount of Ca2+enters into mitochondria and is released to cytosol via NCXmit. NCXmitor NCLX has pivotal roles in Ca2+handling in mitochondria and cytoplasm.