Combined DNA vaccines formulated in DDA enhance protective immunity against tuberculosis
Combined DNA vaccines formulated in DDA enhance protective immunity against tuberculosis
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DOI：
10.1089/1044549041474742
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发表时间：
2004-07-01
影响因子：
3.1
通讯作者：
Zhu, YX
中科院分区：
文献类型：
--
作者：
Cai, H;Tian, X;Zhu, YX
本研究评估了佐剂二甲基二辛基癸基溴化铵 (DDA) 对含有编码结核分枝杆菌抗原 Ag85B、MPT-83 和 ESAT-6 基因的质粒组合诱导的保护性免疫的作用。与盐水中的组合 DNA 疫苗相比，DDA 中的组合 DNA 疫苗导致针对三种纯化抗原的特异性 IgG 和脾 T 细胞衍生的 Th1 型细胞因子γ干扰素 (IFN-γ) 的产生显着增加。 DDA 中的疫苗提高了受结核分枝杆菌 H37Rv 攻击的小鼠的保护效力，通过降低小鼠肺部相对 CFU 计数来衡量。研究表明，用组合 DNA 疫苗免疫的小鼠可以限制肺部和脾脏中结核杆菌的生长。组织病理学分析表明，与对照组相比，接种疫苗的小鼠感染后肺部病理学明显改善。我们认为，我们的抗原与 DDA 制剂的组合可能为结核病预防提供新的见解。
This study evaluated the adjuvant Dimethyldioctyldecyl Ammonium Bromide (DDA) effect on the protective immunity induced by a combination of plasmids containing genes encoding antigens Ag85B, MPT-83, and ESAT-6 from Mycobacterium tuberculosis. The combined DNA vaccines in DDA resulted in significant increases in both specific IgG and splenic T-cell-derived Th1-type cytokine gamma interferon (IFN-gamma) production in response to the three purified antigens when compared to that of combined DNA vaccines in saline. Vaccines in DDA increased the protective efficacy of mice challenged with M. tuberculosis H37Rv as measured by reduced relative CFU counts in their lungs. Mice immunized with the combined DNA vaccines were shown to limit the growth of tubercle bacilli both in lungs and in spleens. Histopathological analyses showed that vaccinated mice had substantially improved postinfection lung pathology relative to the controls. We suggest that our combination of antigens together with DDA formulation may provide a new insight into tuberculosis prevention.