Contribution of Topological Domains and Loop Formation to 3D Chromatin Organization.
Contribution of Topological Domains and Loop Formation to 3D Chromatin Organization.
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DOI：
10.3390/genes6030734
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发表时间：
2015-07-27
期刊：
影响因子：
3.5
通讯作者：
Forné T
中科院分区：
文献类型：
--
作者：
Ea V;Baudement MO;Lesne A;Forné T
最近对3D染色质折叠的研究表明，真核生物基因组是高度区室化的，并且非常动态。本文介绍了真核生物基因组拓扑结构域的最新研究进展，并讨论了拓扑结构域与染色质环形成的关系。CTCF蛋白作为这两个组织水平的中心因子出现，在染色质环形成中具有较强的绝缘作用或较弱的结构作用。边界通过限制特定基因组部分内的接触从而限制TADs内的染色质环形成而直接影响染色质动力学。我们讨论了如何亚染色质动力学，限制到最近描述的统计螺旋构象，可以产生功能的相互作用，通过接触稳定。
Recent investigations on 3D chromatin folding revealed that the eukaryote genomes are both highly compartmentalized and extremely dynamic. This review presents the most recent advances in topological domains’ organization of the eukaryote genomes and discusses the relationship to chromatin loop formation. CTCF protein appears as a central factor of these two organization levels having either a strong insulating role at TAD borders, or a weaker architectural role in chromatin loop formation. TAD borders directly impact on chromatin dynamics by restricting contacts within specific genomic portions thus confining chromatin loop formation within TADs. We discuss how sub-TAD chromatin dynamics, constrained into a recently described statistical helix conformation, can produce functional interactions by contact stabilization.