Characterization of the Neurospora crassa Cell Fusion Proteins, HAM-6, HAM-7, HAM-8, HAM-9, HAM-10, AMPH-1 and WHI-2
Characterization of the Neurospora crassa Cell Fusion Proteins, HAM-6, HAM-7, HAM-8, HAM-9, HAM-10, AMPH-1 and WHI-2
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DOI：
10.1371/journal.pone.0107773
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发表时间：
2014-10-03
期刊：
影响因子：
3.7
通讯作者：
Free, Stephen J.
中科院分区：
文献类型：
--
作者：
Fu, Ci;Ao, Jie;Free, Stephen J.
营养细胞的细胞间通讯及其随后的细胞融合对于丝状真菌的生长、适应性和分化的不同方面至关重要。萌发孢子之间的细胞融合对于早期菌落建立是重要的，而成熟菌落中的菌丝融合促进了资源和细胞器在整个建立的菌落中的移动。在模式丝状真菌粗糙脉孢菌（Neurospora crassa）中，约有50种蛋白质对体细胞间的通讯和融合具有重要作用。遗传，生物化学和显微镜技术被用来表征7个以前很差的特点细胞融合蛋白的功能。HAM-6、HAM-7和HAM-8共享功能特性，并且被提议在相同的信令网络中起作用。我们的数据表明，这些蛋白质可能会形成一个传感器复合物在细胞壁/质膜的MAK-1细胞壁完整性丝裂原活化蛋白激酶（MAPK）途径。我们还证明了HAM-9，HAM-10，AMPH-1和WHI-2具有更一般的功能，并且是正常生长和发育所必需的。MAK-1和MAK-2 MAPK途径的激活状态在缺乏这些蛋白质的突变体中改变。我们建议，这些蛋白质的功能，以协调活动的两个MAPK模块与其他信号通路在细胞融合。
Intercellular communication of vegetative cells and their subsequent cell fusion is vital for different aspects of growth, fitness, and differentiation of filamentous fungi. Cell fusion between germinating spores is important for early colony establishment, while hyphal fusion in the mature colony facilitates the movement of resources and organelles throughout an established colony. Approximately 50 proteins have been shown to be important for somatic cell-cell communication and fusion in the model filamentous fungus Neurospora crassa. Genetic, biochemical, and microscopic techniques were used to characterize the functions of seven previously poorly characterized cell fusion proteins. HAM-6, HAM-7 and HAM-8 share functional characteristics and are proposed to function in the same signaling network. Our data suggest that these proteins may form a sensor complex at the cell wall/plasma membrane for the MAK-1 cell wall integrity mitogen-activated protein kinase (MAPK) pathway. We also demonstrate that HAM-9, HAM-10, AMPH-1 and WHI-2 have more general functions and are required for normal growth and development. The activation status of the MAK-1 and MAK-2 MAPK pathways are altered in mutants lacking these proteins. We propose that these proteins may function to coordinate the activities of the two MAPK modules with other signaling pathways during cell fusion.