Continuous Plankton Recorder in the omics era: from marine microbiome to global ocean observations
Continuous Plankton Recorder in the omics era: from marine microbiome to global ocean observations
复制标题
组学时代的连续浮游生物记录仪：从海洋微生物组到全球海洋观测
DOI：
10.1016/j.copbio.2021.07.016
复制
发表时间：
2022
影响因子：
7.7
通讯作者：
Stern, Rowena
中科院分区：
文献类型：
--
作者：
Vezzulli, Luigi;Martinez-Urtaza, Jaime;Stern, Rowena
要点连续浮游生物记录仪(CPR)调查是世界上最广泛的海洋生物采样计划。EDNA工具与CPR样本的使用开启了全球浮游生物监测的新纪元。需要实施程序将样本污染降至最低，并改进核酸提取和测序。第一次常规部署于1931年的连续浮游生物记录仪(CPR)技术建立了世界上最广泛的海洋生物采样计划。经过90多年的采样，总共覆盖了800万海里，收集了50万个精选样本，CPR调查为海洋研究人员提供了一个信息宝库。由于新的尖端分子技术开始应用于CPR样本，这样的信息可能会呈指数级增长。在这篇综述中，我们的目标是解决基因组革命在CPR应用上的令人兴奋的发展，从海洋微生物组的研究到海洋浮游生物群落，导致全球海洋CPR观测计划的一个新的“数字时代”。
HighlightsThe Continuous Plankton Recorder (CPR) survey is the most extensive, marine biological sampling programme in the world.Use of eDNA tools with CPR samples is opening a new era of global plankton monitoring.Implementation of procedures to minimise sample contamination and improve nucleic acid extraction and sequencing are needed.First routinely deployed in 1931 the Continuous Plankton Recorder (CPR) technology has established the most extensive, marine biological sampling programme in the world. With more than 90 years of sampling, over a total of 8 million nautical miles covered and 500 000 curated samples, the CPR survey provides a gold mine of information available to marine researchers. Such information is likely to exponentially increase thanks to new cutting-edge molecular technologies that are beginning to be applied on CPR samples. In this review we aim to address the exciting developments that the genomic revolution is having on CPR applications from the study of marine microbiome to ocean plankton communities leading to a new ‘digital era’of the global ocean CPR observation programme.