Enzymatic evidence for differences in the placement of Rh antigens within the red cell membrane.
Enzymatic evidence for differences in the placement of Rh antigens within the red cell membrane.
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Rh 抗原在红细胞膜内的位置差异的酶学证据。
作者：
K. Suyama;J. Goldstein
Intact erythrocytes of different Rh genotypes were subjected to various enzyme treatments, the effects of which were monitored by separating the membrane proteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and performing Western blotting using an antibody preparation that recognizes only Rh-related polypeptides. We found that treatment of intact cells with either phospholipase A2 or proteases such as papain did not alter the size of Rh antigen-containing polypeptides. In contrast, phospholipase A2 treatment followed by papain digestion cleaved a fraction of these polypeptides. This cleavage appears, from such digestions of Rh(D) positive and negative cells of different genotypes, to occur solely at the extracellular domain of Rh(D) polypeptide, while the extracellular domains of other Rh antigen-containing polypeptides are unaffected. Digestion of red blood cell ghosts and inside-out vesicles with trypsin showed that Rh(D), (C/c), and (E/e) antigen-containing polypeptides span the lipid bilayer having cytoplasmic domains susceptible to the action of proteases. The size of the cleavage products at the cytoplasmic domain of -D-/-D- cells was found to differ from that of other Rh(D) positive genotypes, due possibly to a difference in folding of Rh(D) polypeptide at its cytoplasmic domain and within the cellular membrane of these cells.
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二维碘肽图谱表明红细胞 Rh D、c 和 E 多肽在结构上同源但不相同。
影响因子：
20.3
作者：
Blanchard,D;Bloy,C;Hermand,P;Cartron,JP;Saboori,AM;Smith,BL;Agre,P
通讯作者：
Agre,P
与红细胞 Rh(D) 抗原相关的 Mr 30,000 整合膜蛋白的纯化和部分表征。
DOI：
--
发表时间：
1987
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Agre,P;Saboori,AM;Asimos,A;Smith,BL
通讯作者：
Smith,BL
细胞外木瓜蛋白酶裂解产生的人红细胞带 3 蛋白肽。
DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Jennings,ML;Adams-Lackey,M;Denney,GH
通讯作者：
Denney,GH
Rh 多肽是一种主要的脂肪酸酰化红细胞膜蛋白。
DOI：
--
发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
deVetten,MP;Agre,P
通讯作者：
Agre,P
从 Rh(D) 阳性和阴性红细胞中纯化的 Mr 32,000 Rh 蛋白的多态性。
DOI：
10.1073/pnas.85.11.4042
发表时间：
1988
影响因子：
11.1
作者：
Saboori,AM;Smith,BL;Agre,P
通讯作者：
Agre,P