Altered distribution of interstitial cells in the guinea pig bladder following bladder outlet obstruction
Altered distribution of interstitial cells in the guinea pig bladder following bladder outlet obstruction
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DOI：
10.1002/nau.20502
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发表时间：
2008-01-01
影响因子：
2
通讯作者：
Kohri, Kenjiro
中科院分区：
文献类型：
--
作者：
Kubota, Yasue;Hashitani, Hikaru;Kohri, Kenjiro
目的：我们研究了膀胱出口梗阻 (BOO) 对豚鼠膀胱间质细胞 (IC) 分布的影响。方法：通过尿动力学研究验证 BOO 动物的膀胱过度活动。对 BOO 膀胱和对照膀胱进行了 Kit 和波形蛋白作为 IC 标记物的免疫组织化学分析。分别通过α-平滑肌肌动蛋白染色和细胞内记录检查逼尿肌平滑肌（DSM）的形态和功能特性。还进行了电子显微镜来表征超微结构。 IC 的形貌。结果：手术后两周，BOO 动物表现出排尿频率增加和排尿量减少。充盈性膀胱测压显示，BOO 膀胱中非排尿收缩频繁发生、排尿收缩之间的间隔缩短以及排尿压力增加。在 BOO 膀胱中，尿路上皮下和浆膜下结缔组织层的厚度增加，而逼尿肌平滑肌 (DSM) 层的厚度受影响较小。 BOO 膀胱浆膜下层中 Kit 或波形蛋白免疫反应性 IC 的数量增加，并且其分布在尿路上皮下层发生改变。 BOO 膀胱中的α-肌动蛋白免疫反应性和 DSM 自发电活动均未改变。 ICs 的特点是有大量的线粒体和细胞膜窝，并且相互之间以及与邻近的 DSM 或神经都有密切的接触。结论：这些结果首次证明了 BOO 豚鼠模型中 IC 数量的增加，并表明 IC 分布的改变可能有助于膀胱过度活动的病理生理学。
Aims: We investigated the effects of bladder outlet obstruction (BOO) on the distribution of interstitial cells (ICs) in the guinea-pig bladder. Methods: Bladder overactivity of BOO animals was validated with urodynamic studies. Immunohistochemical analyses for Kit and vimentin as markers for ICs were performed on both BOO and control bladders. Morphological and functional properties of detrusor smooth muscle (DSM) were examined with a-smooth muscle actin staining and intracellular recording, respectively. Electron microscopy was also carried out to characterize ultrastructural. morphology of ICs. Results: Two weeks after surgery, BOO animals showed an increased voiding frequency and a reduced voiding volume. Filling cystometry demonstrated a frequent incidence of non-voiding contractions, a reduced interval between voiding contractions and an increased voiding pressure in BOO bladders. In BOO bladders, the thickness of suburothelial and subserosal connective tissue layers was increased, whilst that of detrusor smooth muscle (DSM) layer was less affected. Population of Kit or vimentin immunoreactive ICs was increased in subserosal layers, and their distribution was altered in suburotherial layer in BOO bladders. Neither a-actin immunoreactivity nor spontaneous electrical activity of DSM was altered in BOO bladders. ICs were characterized by their numerous mitochondria and caveolae, and had a close contact with each other and with neighboring DSM or nerves. Conclusions: These results demonstrated the increased population of ICs in the BOO guinea-pig model for the first time, and suggest that the altered distribution of ICs may contribute to the pathophysiology of bladder overactivity.