Brummer-dependent lipid mobilization regulates starvation resistance in Nilaparvata lugens
Brummer-dependent lipid mobilization regulates starvation resistance in Nilaparvata lugens
复制标题
布鲁默依赖性脂质动员调节褐飞虱的饥饿抵抗
DOI：
10.1002/arch.21481
复制
发表时间：
2018
影响因子：
2.2
通讯作者：
Lu Kai
中科院分区：
文献类型：
--
作者：
Zhou Jinming;Chen Xia;Yan Jing;You Keke;Yuan Zhineng;Zhou Qiang;Lu Kai
能量稳态是所有生物体的基本特征，需要能量积累，动员和与外部环境交换的波动。在昆虫中，能量动员是在脂肪酶brummer（bmm）的控制下，它通过水解三酰甘油（TAG）中的酯键来调节营养状态。在本研究中，我们研究了BMM在褐飞虱（BPH;Nilaparvata lugens）的脂质动员和饥饿抗性中的作用，褐飞虱是亚洲最重要的水稻害虫之一。在饥饿的BPH中观察到TAG和甘油酯含量的严重下降，而在再喂养后有部分救援。饥饿条件导致Nlbmm的表达水平显著增加，饥饿后补充食物显著降低了Nlbmm的表达。饥饿后的蔗糖拯救显著抑制了Nlbmm的表达，同时引起TAG和甘油酯的积累。通过双链RNA处理敲低Nlbmm延长了饥饿的寿命，而它增加了BPH中TAG和甘油酯的水平。dsNlbmm处理的BPHs的脂肪分解率降低，最终导致饥饿抵抗力增强。这些数据表明，Nlbmm调节能量平衡可能通过控制N. lugens。
Energy homeostasis is an essential characteristic of all organisms, requiring fluctuation in energy accumulation, mobilization, and exchange with the external environment. In insects, energy mobilization is under control of the lipase brummer (bmm), which regulates nutritional status by hydrolyzing the ester bonds in triacylglycerol (TAG). In the present study, we investigated the role of bmm in the lipid mobilization and starvation resistance in the brown planthopper (BPH;Nilaparvata lugens), which is economically one of the most important rice pests in Asia. A severe decrease in TAG and glyceride contents was observed in the starved BPHs, while there was a partial rescue after refeeding. The starvation condition caused a significant increase in the expression levels ofNlbmm, and supplement of food after starvation dramatically reduced theNlbmmexpression. Sucrose rescue after starvation significantly suppressed the expression ofNlbmm, while caused an accumulation of TAG and glyceride. Knockdown ofNlbmmby double‐stranded RNA treatment extended the lifespan to starvation, whereas it increased the level of TAG and glyceride in the BPHs. The decreased lipolysis rate by dsNlbmm‐treated BPHs eventually resulted in increase of starvation resistance. These data demonstrated that the regulation of energy homeostasis byNlbmmaffects starvation resistance, probably through lipid mobilization control inN. lugens.