Divergently expressed RNA identification and interaction prediction of long non-coding RNA and mRNA involved in Hu sheep hair follicle
Divergently expressed RNA identification and interaction prediction of long non-coding RNA and mRNA involved in Hu sheep hair follicle
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湖羊毛囊中长链非编码RNA和mRNA的差异表达RNA鉴定及相互作用预测
DOI：
10.1038/s41598-019-43854-8
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发表时间：
2019-05-13
影响因子：
4.6
通讯作者：
Sun, Wei
中科院分区：
文献类型：
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作者：
Lv, Xiaoyang;Gao, Wen;Sun, Wei
毛囊是胡羊花纹形成的基础。本研究采用全转录组测序技术筛选羔羊皮三种波型之间差异表达的长链非编码rna （lncRNAs）。本研究选取2日龄湖羊全同胞小波、中波和大波3组，采集湖羊背部毛囊组织。采用全转录组测序技术分析LncRNA和mRNA的表达谱。在大与中、中与小、大与小之间分别筛选出33、31、41个差异表达的lncrna。通过RNA-seq分析，在大与中、中与小、大与小之间分别发现458、481、498个差异表达mrna。随机选取的16个lncrna和mrna的qRT-PCR结果与测序结果相似。lncRNA与mRNA表达的相关性分析显示，毛囊中可能富集了Wnt、mTOR、Notch信号通路等多种lncRNA。我们的研究结果有助于挖掘毛囊中的mrna和lncrna，为进一步研究模式形成机制提供基础。
Hair follicles are the basis of the formation of Hu sheep pattern. This study was to employ whole transcriptome sequencing to screen differentially expressed long non-coding RNAs (lncRNAs) between three wave patterns in lambskin. In this study, three groups of 2-day-old Hu sheep were selected from full-sib individuals that included small, medium, and large waves, and hair follicle tissues were collected from dorsal side of Hu sheep. LncRNA and mRNA expression profiles were analyzed by whole transcriptome sequencing technology. 33, 31, and 41 differentially expressed lncRNAs were selected between large and medium, medium and small, and large and small, respectively. 458, 481, and 498 differentially expressed mRNAs were found between large and medium, medium and small, and large and small, respectively, by RNA-seq analysis. qRT-PCR results of 16 randomly selected lncRNAs and mRNAs were similar to the sequencing results. Correlation analysis of lncRNA and mRNA expression showed that, several lncRNAs may be enriched for hair follicle such as Wnt, mTOR, Notch signaling pathways. Our results aid in excavation of mRNAs and lncRNAs in hair follicle, and providing a basis for future study on pattern formation mechanisms.