CLONING OF A NOVEL TYPE-II SERINE THREONINE KINASE RECEPTOR THROUGH INTERACTION WITH THE TYPE-I TRANSFORMING GROWTH-FACTOR-BETA RECEPTOR
CLONING OF A NOVEL TYPE-II SERINE THREONINE KINASE RECEPTOR THROUGH INTERACTION WITH THE TYPE-I TRANSFORMING GROWTH-FACTOR-BETA RECEPTOR
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DOI：
10.1074/jbc.270.10.5625
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发表时间：
1995-03-10
影响因子：
4.8
通讯作者：
MOSES, HL
中科院分区：
文献类型：
--
作者：
KAWABATA, M;CHYTIL, A;MOSES, HL
转化生长因子-β（TGF-β）超家族包括参与多种生物过程的许多分子。该家族的几个成员的特异性受体已被分子鉴定，形成一类新的跨膜丝氨酸/苏氨酸激酶受体。I型和II型受体在物理上和功能上相互作用，从而合作产生细胞内信号。酵母双杂交系统用于鉴定可以与I型TGF-β受体的胞质区域相互作用的蛋白质。鉴定的蛋白质之一编码一种新的推定的丝氨酸/苏氨酸激酶受体。序列分析表明该分子属于II型受体。然而，这种受体与其他II型受体的不同之处在于具有非常长的C-末端尾区。成人组织中的表达模式与其他已知的II型受体不同;它在心脏和肝脏中高度表达。在酵母系统中，I型和II型受体的不同组合的细胞质区域异源二聚化，为可能存在的TGF-β超家族的许多配体的大量丝氨酸/苏氨酸激酶受体提供了新的克隆策略。
The transforming growth factor-beta (TGF-beta) superfamily comprises a number of molecules that are involved in a wide variety of biological processes. Specific receptors for several members of this family have been molecularly identified, forming a new category of transmembrane serine/threonine kinase receptors. The type I and type II receptor interact both physically and functionally, thereby cooperating to generate intracellular signals. The yeast two-hybrid system was used to identify proteins that can interact with the cytoplasmic region of the type I TGF-beta receptor. One of the proteins identified encodes a novel putative serine/threonine kinase receptor. Sequence analysis suggests that this molecule belongs to the type II receptor class. This receptor, however, is distinct from other type II receptors in having an extraordinarily long C-terminal tail region. The pattern of expression in adult tissues is different from that of other known type II receptors; it is highly expressed in heart and liver. In the yeast system, the cytoplasmic regions of different combinations of type I and type II receptors heterodimerize, providing a new cloning strategy for the large number of serine/threonine kinase receptors likely to exist for the many ligands of the TGF-beta superfamily.