Functional stratification of the Spitzenkörper of Neurospora crassa
Functional stratification of the Spitzenkörper of Neurospora crassa
复制标题
粗糙脉孢菌 Spitzenkörper 的功能分层
DOI：
10.1111/j.1365-2958.2009.06917.x
复制
发表时间：
2009
影响因子：
3.6
通讯作者：
M. Riquelme
中科院分区：
文献类型：
--
作者：
Jorge Verdín;S. Bartnicki;M. Riquelme
GS‐1 (ncu04189) is a protein required for the synthesis of β‐1,3‐glucan in Neurospora crassa. As chitin, β‐1,3‐glucan is a morphogenetically relevant component of the fungal cell wall. Previously, we showed that chitin synthases are delivered to the growing hyphal tip of N. crassa by secretory microvesicles that follow an unconventional route and accumulate in the core of the Spitzenkörper (Spk). Tagged with the green fluorescent protein (GFP), GS‐1 accumulated in the hyphal apex forming a dynamic and pleomorphic ring‐like structure (‘Spitzenring’) that corresponded to the Spk outer macrovesicular stratum and surrounded the inner core of chitin synthase‐containing microvesicles. TIRF microscopy revealed that GS‐1‐GFP reached the hyphal apex as a population of heterogeneous‐size particles that moved along defined paths. On sucrose density gradients, GS‐1‐associated particles mainly sedimented in a high density range 1.1272–1.2124 g ml−1. Clearly, GS‐1 and chitin synthases of N. crassa are contained in two different types of secretory vesicles that accumulate in different strata of the Spk, a differentiation presumably related to the spatial control of cell‐wall synthesis.
DOI：
10.1016/s1087-1845(02)00506-6
发表时间：
2002-11
期刊：
Fungal genetics and biology : FG & B
影响因子：
--
作者：
M. Riquelme;R. Roberson;D. McDaniel;S. Bartnicki-García
通讯作者：
M. Riquelme;R. Roberson;D. McDaniel;S. Bartnicki-García
影响因子：
3
作者：
Freitag, M;Hickey, PC;Read, ND
通讯作者：
Read, ND