CENP-H, a constitutive centromere component, is required for centromere targeting of CENP-C in vertebrate cells
CENP-H, a constitutive centromere component, is required for centromere targeting of CENP-C in vertebrate cells
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DOI：
10.1093/emboj/20.16.4603
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发表时间：
2001-08-15
期刊：
影响因子：
11.4
通讯作者：
Ikemura, T
中科院分区：
文献类型：
--
作者：
Fukagawa, T;Mikami, Y;Ikemura, T
CENP - H最近被发现是着丝粒的一个组成成分，在整个细胞周期中与CENP - A和CENP - C共定位。然而，其确切功能仍知之甚少。我们通过在鸡DT40细胞系中产生一个条件性功能缺失突变体来研究CENT - H在着丝粒功能和组装中的作用。在缺乏CENP - H的情况下，观察到细胞在中期周期停滞，这与着丝粒功能丧失一致。对CENP - H缺陷细胞的免疫细胞化学分析表明，CENP - H对于CENP - C（而非CENP - A）定位到着丝粒是必需的。这些发现表明，脊椎动物细胞中的着丝粒组装是以一种分级方式进行的，其中着丝粒特异性组蛋白CENP - A的定位是一个早期事件，它独立于CENP - C和CENP - H发生。
CENP-H has recently been discovered as a constitutive component of the centromere that co-localizes with CENP-A and CENP-C throughout the cell cycle. The precise function, however, remains poorly understood. We examined the role of CENT-H in centromere function and assembly by generating a conditional loss-of-function mutant in the chicken DT40 cell line. In the absence of CENP-H, cell cycle arrest at metaphase, consistent with loss of centromere function, was observed. Immunocytochemical analysis of the CENP-H-deficient cells demonstrated that CENP-H is necessary for CENP-C, but not CENP-A, localization to the centromere. These findings indicate that centromere assembly in vertebrate cells proceeds in a hierarchical manner in which localization of the centromere-specific histone CENP-A is an early event that occurs independently of CENP-C and CENP-H.