The prokaryotic and fungal communities of oat rhizosphere responded contrastingly to different irrigation regimes at pre- and post-anthesis stages
The prokaryotic and fungal communities of oat rhizosphere responded contrastingly to different irrigation regimes at pre- and post-anthesis stages
复制标题
燕麦根际原核和真菌群落在花前和花后阶段对不同灌溉方式的反应截然不同
DOI：
10.1016/j.apsoil.2022.104490
复制
发表时间：
2022-08-01
影响因子：
4.8
通讯作者：
Bai, Wenming
中科院分区：
文献类型：
--
作者：
Bai, Ren;Hou, Longyu;Bai, Wenming
植物从营养生长期向生殖生长期的转变和土壤水分状况的变化会通过影响光合作用产物向根的分配来改变根际微生物群落的反馈。然而，不同的根际微生物区系在不同灌溉制度下对抗旱作物生长阶段的反应却知之甚少。为了解决这些问题，我们研究了灌溉制度对中国东北部半干旱地区燕麦开花前和开花后根际原核生物和真菌群落的影响。利用Illumina高通量测序技术对燕麦根际原核生物和真菌群落特征进行了研究。结果表明，原核生物多样性主要受燕麦生育期的调控，而真菌多样性主要受灌溉制度的影响。同样，所有原核生物门对燕麦生长阶段的响应显著，而不是灌溉制度，而真菌类群的组成在很大程度上受灌溉制度的调节。旱地耕作条件下鉴定出的主要致病镰刀菌属的比例比灌溉处理高出约2-3倍。此外，功能预测表明，原核生物和真菌的不同反应特征归因于它们的功能组合，这与燕麦的生长阶段和土壤水分条件有关。然而，原核生物网络的性质主要受灌溉制度的影响，而真菌网络则受燕麦生长的调控，这意味着微生物多样性和群落稳定性的不同决定机制。结果表明，根际原核生物群落和真菌群落对燕麦生长阶段和土壤水分历史的响应不同，这突出了灌溉对半干旱地区根际微生物调控的重要性。
The transition from the vegetative stage to reproductive stage of plants and variations in soil water regimes can alter the community feedbacks of rhizosphere microbiomes by affecting the partitioning of photosynthates to roots. However, little is known about how different rhizosphere microbiota respond to the growth stages of drought-resistant crops under different irrigation regimes. To address these issues, we investigated the effects of irrigation regimes on the rhizosphere prokaryotic and fungal communities at the pre-and post-anthesis stages of oats in a semi-arid region of northeastern China. The prokaryotic and fungal community traits of the oat rhizosphere were characterized by Illumina high-throughput sequencing. The results revealed that the pro-karyotic diversity was primarily regulated by oat growth stage, while the fungal diversity was mainly increased by irrigation regimes. Similarly, all prokaryotic phyla responded significantly to oat growth stages rather than irrigation regimes, while the composition of the fungal taxa was largely regulated by irrigation regimes. Approximately 2-3-fold higher proportions of the predominating pathogenic Fusarium genus were identified under dryland farming conditions than under irrigation treatment. In addition, functional predictions suggested that the contrasting responsive traits of prokaryotes and fungi were attributed to their functional guilds, which were related to oat growth stages and soil water conditions. However, the properties of the prokaryotic networks mainly responded to irrigation regimes while the fungal networks were regulated by oat growth, implying distinct determination mechanisms for microbial diversity and community stability. Our results indicate that rhizosphere prokaryotic and fungal communities differentially responded to oat growth stages and soil water history, highlighting the significance of irrigation in manipulating rhizosphere microbes in semi-arid regions.