Genetic control of rice blast resistance in the durably resistant cultivar Gumei 2 against multiple isolates
Genetic control of rice blast resistance in the durably resistant cultivar Gumei 2 against multiple isolates
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DOI：
10.1007/s00122-005-1971-2
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发表时间：
2005-06-01
影响因子：
5.4
通讯作者：
Zhuang, JY
中科院分区：
文献类型：
--
作者：
Wu, JL;Fan, YY;Zhuang, JY
为了进一步了解水稻品种谷梅2号抗稻瘟病的遗传控制，从而促进这一持久抗稻瘟病品种的利用，我们研究了中156/谷梅2号组合的304个重组自交系和一个包含181个标记的连锁图谱。对谷梅2号对5个稻瘟病菌株的抗性分离分析表明，谷梅2号的完全抗性由1对基因簇和3对独立遗传的主效基因控制。该基因簇位于第6号染色体上，包括先前定位的两个基因，Pi 25（t），抗中国稻瘟病分离物92-183（小种ZC 15）和Pi 26（t），抗菲律宾稻瘟病分离物Ca 89（谱系4），以及抗菲律宾稻瘟病分离物92330-5（谱系17）的基因。在赋予对菲律宾分离物V86013（谱系15）和C923-39（谱系46）的抗性的两个基因中，我们将一个鉴定为Pi 26（t），并将另一个定位到Pib所在的2号染色体的远端。我们使用了三个组成部分的部分稻瘟病抗性，百分比患病叶面积（DLA），病斑数和病斑大小，都在温室中测量，来衡量的敏感程度分离Ca 89和C923-39，随后确定了9个和8个数量性状位点（QTL），分别。上位性被确定在对Ca 89的部分抗性中起重要作用。利用在稻瘟病圃中对感病株系的DLA测定，共检测到6个QTL。对Ca 89和C923-39的部分抗性分别检测到不同的QTL，但田间部分抗性QTL占多数。研究结果表明，谷梅2号的抗瘟性受多对主效基因和微效基因控制，且存在上位性效应。
To further our understanding of the genetic control of blast resistance in rice cultivar Gumei 2 and, consequently, to facilitate the utilization of this durably blast-resistant cultivar, we studied 304 recombinant inbred lines of indica rice cross Zhong 156/Gumei 2 and a linkage map comprising 181 markers. An analysis of segregation for resistance against five isolates of rice blast suggested that one gene cluster and three additional major genes that are independently inherited are responsible for the complete resistance of Gumei 2. The gene cluster was located to chromosome 6 and includes two genes mapped previously, Pi25(t), against Chinese rice blast isolate 92-183 (race ZC15) and Pi26(t) against Philippine rice blast isolate Ca89 (lineage 4), and a gene for resistance against Philippine rice blast isolate 92330-5 (lineage 17). Of the two genes conferring resistance against the Philippine isolates V86013 (lineage 15) and C923-39 (lineage 46), we identified one as Pi26(t) and mapped the other onto the distal end of chromosome 2 where Pib is located. We used three components of partial blast resistance, percentage diseased leaf area (DLA), lesion number and lesion size, all measured in the greenhouse, to measure the degree of susceptibility to isolates Ca89 and C923-39 and subsequently identified nine and eight quantitative trait loci (QTLs), respectively. Epistasis was determined to play an important role in partial resistance against Ca89. Using DLA measured on lines susceptible in a blast nursery, we detected six QTLs. While different QTLs were detected for partial resistance to Ca89 and C923-39, respectively, most were involved in the partial resistance in the field. Our results suggest that the blast resistance in Gumei 2 is controlled by multiple major genes and minor genes with epistatic effects.