Compositional analysis of the glycosaminoglycan family in velvet antlers of Sika deer (Cervus nippon) at different growing stages.
Compositional analysis of the glycosaminoglycan family in velvet antlers of Sika deer (Cervus nippon) at different growing stages.
复制标题
梅花鹿不同生长阶段鹿角糖胺聚糖家族的组成分析。
DOI：
10.1007/s10719-019-09859-1
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Tamura JI.
中科院分区：
文献类型：
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作者：
Takeda-Okuda N;Mizumoto S;Zhang Z;Kim SK;Lee CH;Jeon BT;Hosaka YZ;Kadomatsu K;Yamada S;Tamura JI.
从不同生长阶段（黄鹤、安氏和桑塔霍）的西卡鹿茸中分离出糖胺聚糖（GAG），并对其含量和硫酸化模式进行了分析。用软骨素酶ABC、ACI、肝素酶-I和-III以及角质素酶-II将GAG消化成硫酸软骨素（CS）、硫酸皮肤素（DS）、透明质酸、硫酸乙酰肝素（HS）和硫酸角质素的相应重复二糖。软骨组织含有高浓度的CS-DS，4-和6-硫酸盐的比例几乎相等，而4-硫酸盐型CS-DS主要占据骨化组织，但浓度较低。HS的高O-和N-硫酸化程度对应于高骨化。鹿茸中CS-DS含量的动态变化以及CS-DS和HS组成的变化与鹿茸的矿化密切相关。
Glycosaminoglycans (GAG) from the velvet antlers of Sika deer (Cervus nippon) at the different growing stages (Fukurozuno, Anshi, and Santajo) of bred and wild deer were isolated and their concentrations and sulfation patterns were analyzed. GAG were digested with chondroitinase ABC, ACI, heparinase-I and -III, and keratanase-II into the corresponding repeating disaccharides of chondroitin sulfate (CS), dermatan sulfate (DS), hyaluronan, heparan sulfate (HS), and keratan sulfate. Cartilaginous tissues contained CS-DS at high concentrations with an almost equal ratio of 4- and 6-sulfates, while 4-sulfate-type CS-DS predominantly occupied ossified tissues, but at low concentrations. HighO- andN-sulfation degrees of HS correspond to high ossification. Dynamic quantitative changes in CS-DS and compositional changes in CS-DS and HS were closely associated with the mineralization of deer antlers.