Genetic and phenotypic analysis of alleles of the Drosophila chromosomal JIL-1 kinase reveals a functional requirement at multiple developmental stages.
Genetic and phenotypic analysis of alleles of the Drosophila chromosomal JIL-1 kinase reveals a functional requirement at multiple developmental stages.
复制标题
果蝇染色体 JIL-1 激酶等位基因的遗传和表型分析揭示了多个发育阶段的功能需求。
DOI：
10.1093/genetics/165.3.1341
复制
发表时间：
2003
期刊：
影响因子：
3.3
通讯作者：
Johansen,KristenM
中科院分区：
文献类型：
--
作者：
Zhang,Weiguo;Jin,Ye;Ji,Yun;Girton,Jack;Johansen,Jørgen;Johansen,KristenM
In this study we provide a cytological and genetic characterization of theJIL-1locus in Drosophila. JIL-1 is an essential chromosomal tandem kinase and inJIL-1null animals chromatin structure is severely perturbed. Using a range ofJIL-1hypomorphic mutations, we show that they form an allelic series. JIL-1 has a strong maternal effect and JIL-1 activity is required at all stages of development, including embryonic, larval, and pupal stages. Furthermore, we identified a new allele ofJIL-1, JIL-1h9, that encodes a truncated protein missing COOH-terminal sequences. Remarkably, the truncated JIL-1 protein can partially restore viability without rescuing the defects in polytene chromosome organization. This suggests that sequences within this region of JIL-1 play an important role in establishing and/or maintaining normal chromatin structure. By analyzing the effects ofJIL-1mutations we provide evidence that JIL-1 function is necessary for the normal progression of several developmental processes at different developmental stages such as oogenesis and segment specification. We propose that JIL-1 may exert such effects by a general regulation of chromatin structure affecting gene expression.
