An unusual structure at one end of potato potyvirus particles
An unusual structure at one end of potato potyvirus particles
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DOI：
10.1016/j.jmb.2005.12.021
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发表时间：
2006-03-17
影响因子：
5.6
通讯作者：
Taliansky, ME
中科院分区：
文献类型：
--
作者：
Torrance, L;Andreev, IA;Taliansky, ME
马铃薯Y病毒(PVY)和马铃薯A病毒(PVA)马铃薯Y病毒的颗粒是螺旋结构的细丝，被认为含有单一类型的外壳蛋白亚基。通过电子显微镜对负染病毒粒子的检查发现，弯曲的杆状颗粒没有明显的末端颗粒，含有额外的少量蛋白质组分，形成稳定的复合体，介导颗粒的分解、移动或通过载体传输。其中一些信息是通过原子力显微镜等成像技术获得的。用原子力显微镜和免疫金标记法对PVY和PVA颗粒进行观察。我们的结果表明，一些马铃薯Y病毒颗粒在病毒颗粒的一端含有一个突出的尖端，这可能与病毒RNA的5‘端有关。LIP含有病毒编码蛋白、基因组连接蛋白和辅助成分蛋白。讨论了末端末端结构的组成及其在病毒生物学中的可能作用。(C)2005爱思唯尔有限公司。保留所有权利。
The particles of potato virus Y (PVY) and potato virus A (PVA) potyviruses are helically constructed filaments that are thought to contain a single type of coat protein subunit. Examination of negatively stained virions by electron microscopy reveals flexuous rod-shaped particles with no obvious terminal particles incorporate additional minor protein components, which form stable complexes that mediate particle disassembly, movement or transmission by vectors. Some of this information has been obtained using imaging techniques such as atomic force microscopy. The particles of PVY and PVA were examined by atomic force microscopy and immunogold labelling electron microscopy. Our results show that some of the potyvirus particles contain a protruding tip at one end of the virus particles, which is presumably associated with the 5'-end of viral RNA. The lip contains the virus-encoded proteins genome-linked protein and helper-component proteinase. The composition and possible roles of the terminal tip structures in virus biology are discussed. (c) 2005 Elsevier Ltd. All rights reserved.