TRAPPC13 Is a Novel Target of Mesorhizobium amorphae Type III Secretion System Effector NopP
TRAPPC13 Is a Novel Target of Mesorhizobium amorphae Type III Secretion System Effector NopP
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DOI：
10.1094/mpmi-12-20-0354-fi
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发表时间：
2021-05-01
影响因子：
3.5
通讯作者：
Wei，Gehong
中科院分区：
文献类型：
--
作者：
Liu，Dongying;Luo，Yantao;Wei，Gehong
与病原菌类似，根瘤菌可以直接将效应蛋白注入宿主细胞，通过III型分泌系统(T3SS)促进感染。结瘤外蛋白P(NOPP)是根瘤菌特有的T3SS效应蛋白，在根瘤菌-豆科植物共生系统的建立中起着不同的作用。在这里，我们证明了在感染的早期阶段，NOPP是通过GS0123的T3SS-I分泌的，它的缺失降低了根瘤固氮酶的活性。假无菌结节。运输蛋白颗粒复合亚单位13亚基样蛋白(TRAPPC13)已被鉴定为NOPP靶蛋白INR。筛选酵母双杂交文库。通过双分子荧光互补和免疫共沉淀实验验证了NOPP与TRAPPC13之间的物理相互作用。此外，亚细胞定位分析表明，NOPP及其靶标TRAPPC13均共定位于质膜上。与接种GS0123的R。在接种ΔNOPP后36h，一些与细胞壁重塑和植物天然免疫相关的基因在根中表达下调。结果表明，NOPP INM.AmorphaeCCNWGS0123在INR的多个进程中起作用。在感染的早期阶段，TRAPPC13可以作为NOPP靶标参与这一过程。版权所有©2021作者(S)。这是一篇在CC BY-NC-ND 4.0国际许可证下分发的开放获取文章。
Similar to pathogenic bacteria, rhizobia can inject effector proteins into host cells directly to promote infection via the type III secretion system (T3SS). Nodulation outer protein P (NopP), a specific T3SS effector of rhizobia, plays different roles in the establishment of multiple rhizobia-legume symbiotic systems.Mesorhizobium amorphaeCCNWGS0123 (GS0123), which infectsRobinia pseudoacaciaspecifically, secretes several T3SS effectors, including NopP. Here, we demonstrate that NopP is secreted through T3SS-I of GS0123 during the early stages of infection, and its deficiency decreases nodule nitrogenase activity ofR. pseudoacacianodules. A trafficking protein particle complex subunit 13–like protein (TRAPPC13) has been identified as a NopP target protein inR. pseudoacaciaroots by screening a yeast two-hybrid library. The physical interaction between NopP and TRAPPC13 is verified by bimolecular fluorescence complementation and coimmunoprecipitation assays. In addition, subcellular localization analysis reveals that both NopP and its target, TRAPPC13, are colocalized on the plasma membrane. Compared with GS0123-inoculatedR. pseudoacaciaroots, some genes associated with cell wall remodeling and plant innate immunity down-regulated in ΔnopP-inoculated roots at 36 h postinoculation. The results suggest that NopP inM. amorphaeCCNWGS0123 acts in multiple processes inR. pseudoacaciaduring the early stages of infection, and TRAPPC13 could participate in the process as a NopP target.Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.