A microfluidic device with a linear temperature gradient for parallel and combinatorial measurements
A microfluidic device with a linear temperature gradient for parallel and combinatorial measurements
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DOI：
10.1021/ja017625x
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发表时间：
2002-04-24
影响因子：
15
通讯作者：
Cremer, PS
中科院分区：
文献类型：
--
作者：
Mao, HB;Yang, TL;Cremer, PS
在化学和生物科学中，需要获得作为温度函数的组合和基于数组的数据的方法。目前使用标准井板格式很难使用温度作为变量，因为将每口井保持在不同的温度非常不方便。然而，在微流体中，由于所涉及的鳞片长度较短，情况非常不同。在这篇文章中，它展示了如何在几十个平行的微流控通道上同时产生简单的线性温度梯度。这一结果被用来快速获得催化反应的活化能、脂膜的熔点转变以及半导体纳米晶体探针的荧光量子产率随温度的变化曲线。这里开发的方法可以很容易地扩展到蛋白质结晶、相图测量、化学反应优化或多变量实验。
Methods for obtaining combinatorial and array-based data as a function of temperature are needed in the chemical and biological sciences. It is presently quite difficult to employ temperature as a variable using standard wellplate formats simply because it is very inconvenient to keep each well at a distinct temperature. In microfluidics, however, the situation is very different due to the short length scales involved. In this article, it is shown how a simple linear temperature gradient can be generated across dozens of parallel microfluidic channels simultaneously. This result is exploited to rapidly obtain activation energies from catalytic reactions, melting point transitions from lipid membranes, and fluorescence quantum yield curves from semiconductor nanocrystal probes as a function of temperature. The methods developed here could quite easily be extended to protein crystallization, phase diagram measurements, chemical reaction optimization, or multivariable experiments.