Molecular Epidemiology of Plasmid-Mediated Types 1 and 3 Fimbriae Associated with Biofilm Formation in Multidrug Resistant Escherichia coli from Diseased Food Animals in Guangdong, China.
Molecular Epidemiology of Plasmid-Mediated Types 1 and 3 Fimbriae Associated with Biofilm Formation in Multidrug Resistant Escherichia coli from Diseased Food Animals in Guangdong, China.
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DOI：
10.1128/spectrum.02503-21
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发表时间：
2022-10-26
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
--
作者：
肠杆菌科中的 1 型和 3 型菌毛在细菌生理学中发挥着多种作用，包括附着、侵袭、细胞运动以及生物膜形成和尿路感染。在此，我们研究了来自患病食用动物的 1753 个非重复肠杆菌科细菌中质粒介导的 1 型和 3 型菌毛的流行和传播。我们确定了 123 个（7.01%）强生物膜产生者，全部被确定为大肠杆菌。对 43 个选定的强大生物膜生产者的全基因组测序分析显示，它们含有多种 ARG，包括 ESBL、PMQR 和 mcr-1。分别在 123 个强生物膜分离株中的 43 个（34.96%）和 7 个（5.7%）中鉴定出分别编码 1 型和 3 型菌毛的基因簇 mrkABCDF 和 fimACDH。这两个操纵子能够赋予大肠杆菌弱生物膜形成实验室菌株强大的生物膜形成能力。质粒分析表明，mrk 和 fim 操纵子被发现与 ARG 共存，并且主要位于具有相似主链的 IncX1 和 IncFII 质粒上。根据存档的 WGS 数据，mrkABCDF 操纵子存在于所有 9457 株肺炎克雷伯菌中，并且与来自不同来源和国家的 6 种肠杆菌科细菌的 8 种复制子类型和染色体上的操纵子具有高度同源性。相比之下，fimACDH操纵子存在于大多数阴沟肠杆菌中（62.15%），并且与仅少数质粒和肠杆菌科物种具有较高的同源性。这是第一份关于肠杆菌科中质粒编码的 1 型和 3 型菌毛的流行、传播和同源性的综合报告。我们的研究结果表明，质粒编码的 mrkABCDF 和 fimACDH 是增强大肠杆菌和这两个操纵子中生物膜形成的主要因素，特别是 mrk 可以作为潜在的抗生物膜靶点。重要性 生物膜使细菌能够耐受消毒剂和抗菌剂以及哺乳动物宿主的防御，因此在临床上难以治疗。大多数有关生物膜相关感染的研究通常集中于染色体生物膜相关因素，包括形成生物膜的肠杆菌的 1 型和 3 型菌毛。然而，肠杆菌科中1型和3型移动菌毛的传播和同源性在很大程度上尚不清楚。研究结果表明，mrkABCDF 编码的质粒编码 3 型菌毛和 fimACDH 编码的 1 型菌毛是增强来自患病食品生产动物的强生物膜大肠杆菌中生物膜形成的主要因素。此外，在氨基酸序列上具有高度同源性的mrk操纵子存在于各种复制子类型的质粒上以及来自多个来源和国家的不同肠杆菌科物种的染色体上。这些发现提供了有关肠杆菌科细菌中 1 型和 3 型移动菌毛传播的重要信息，表明了潜在的抗菌膜目标。
Types 1 and 3 fimbriae in Enterobacteriaceae play versatile roles in bacterial physiology including attachment, invasion, cell motility as well as with biofilm formation and urinary tract infections. Herein, we investigated the prevalence and transmission of plasmid-mediated types 1 and 3 fimbriae from 1753 non-duplicate Enterobacteriaceae from diseased food Animals. We identified 123 (7.01%) strong biofilm producers and all was identified as E. coli. WGS analysis of 43 selected strong biofilm producers revealed that they harbored multiple ARGs, including ESBLs, PMQR and mcr-1. The gene clusters mrkABCDF and fimACDH encoding types 1 and 3 fimbriae, respectively, were identified among 43 (34.96%) and 7 (5.7%) of 123 strong biofilm isolates, respectively. These two operons were able to confer strong biofilm-forming ability to an E. coli weak-biofilm forming laboratory strain. Plasmid analysis revealed that mrk and fim operons were found to co-exist with ARGs and were primarily located on IncX1 and IncFII plasmids with similar backbones, respectively. mrkABCDF operons was present in all of 9457 Klebsiella pneumoniae using archived WGS data, and shared high homology to those on plasmids of 8 replicon types and chromosomes from 6 Enterobacteriaceae species from various origins and countries. In contrast, fimACDH operons was present in most of Enterobacter cloacae (62.15%), and shared high homology to those with only a small group of plasmids and Enterobacteriaceae species. This is the first comprehensive report of the prevalence, transmission and homology of plasmid-encoded type 1 and 3 fimbriae among the Enterobacteriaceae. Our findings indicated that plasmid-encoded mrkABCDF and fimACDH were major contributors to enhanced biofilm formation among E. coli and these two operons, in particular mrk could be as a potential anti-biofilm target. IMPORTANCE Biofilms allow bacteria to tolerate disinfectants and antimicrobials, as well as mammalian host defenses, and are therefore difficult to treat clinically. Most research concerning biofilm-related infections is typically focused on chromosomal biofilm-associated factors, including types 1 and 3 fimbriae of biofilm-forming Enterobacterium. However, the transmission and homology of the mobile types 1 and 3 fimbriae among Enterobacteriaceae is largely unknown. The findings revealed that the plasmid-encoded type 3 fimbriae encoded by mrkABCDF and type 1 fimbriae encoded by fimACDH were major contributors to enhancing biofilm formation among strong biofilm E. coli from diseased food producing animals. Additionally, mrk operon with high homology at an amino acid sequence was present both on plasmids of various replicon types and on chromosomes from diverse Enterobacteriaceae species from numerous origins and countries. These findings provide important information on the transmission of the mobile types 1 and 3 fimbriae among Enterobacteriaceae, indicating a potential antibiofilm target.
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影响因子：
4.9
作者：
Hansen, LH;Johannesen, E;Sorensen, SJ
通讯作者：
Sorensen, SJ
DOI：
10.1093/jac/dkaa345
发表时间：
2020-12-01
期刊：
The Journal of antimicrobial chemotherapy
影响因子：
--
作者：
Bortolaia V;Kaas RS;Ruppe E;Roberts MC;Schwarz S;Cattoir V;Philippon A;Allesoe RL;Rebelo AR;Florensa AF;Fagelhauer L;Chakraborty T;Neumann B;Werner G;Bender JK;Stingl K;Nguyen M;Coppens J;Xavier BB;Malhotra-Kumar S;Westh H;Pinholt M;Anjum MF;Duggett NA;Kempf I;Nykäsenoja S;Olkkola S;Wieczorek K;Amaro A;Clemente L;Mossong J;Losch S;Ragimbeau C;Lund O;Aarestrup FM
通讯作者：
Aarestrup FM
影响因子：
1.7
作者：
Bankevich, Anton;Nurk, Sergey;Pevzner, Pavel A.
通讯作者：
Pevzner, Pavel A.
影响因子：
14.9
作者：
Letunic, Ivica;Bork, Peer
通讯作者：
Bork, Peer
影响因子：
2.8
作者：
Burmolle, Mette;Bahl, Martin Lain;Hansen, Lars Hestbjerg
通讯作者：
Hansen, Lars Hestbjerg