Effects of a synthetic bioactive peptide on neurite growth and nerve growth factor release in chondroitin sulfate hydrogels.
Effects of a synthetic bioactive peptide on neurite growth and nerve growth factor release in chondroitin sulfate hydrogels.
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DOI：
10.4161/biom.17849
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发表时间：
2011-10
期刊：
影响因子：
--
通讯作者：
Panitch A
中科院分区：
文献类型：
--
作者：
Conovaloff AW;Beier BL;Irazoqui PP;Panitch A
先前的工作已经揭示了硫酸软骨素水凝胶中背根神经节神经突的强劲生长。在目前的工作中，确定了添加合成的生物活性肽是否可以通过增强生长因子的结合和螯合来增加这些基质中的神经突生长。光漂白研究后的荧光恢复显示，除了肽减缓神经生长因子在硫酸软骨素凝胶中的扩散，但在透明质酸的对照凝胶中没有。此外，鸡背根神经节在透明质酸或硫酸软骨素凝胶中的培养物仅在添加肽后显示硫酸软骨素凝胶中的生长增强。总之，这些结果表明，这种肽和硫酸软骨素之间的协同神经生长因子结合活性。
Previous work has revealed robust dorsal root ganglia neurite growth in hydrogels of chondroitin sulfate. In the current work, it was determined whether addition of a synthetic bioactive peptide could augment neurite growth in these matrices via enhanced binding and sequestering of growth factors. Fluorescence recovery after photobleaching studies revealed that addition of peptide slowed nerve growth factor diffusivity in chondroitin sulfate gels, but not in control gels of hyaluronic acid. Furthermore, cultures of chick dorsal root ganglia in gels of hyaluronic acid or chondroitin sulfate revealed enhanced growth in chondroitin sulfate gels only upon addition of peptide. Taken together, these results suggest a synergistic nerve growth factor-binding activity between this peptide and chondroitin sulfate.