Composition, spatial distribution, and diversity of the bacterial communities in the rumen of cows fed different forages
Composition, spatial distribution, and diversity of the bacterial communities in the rumen of cows fed different forages
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DOI：
10.1111/j.1574-6941.2010.00977.x
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发表时间：
2010-12-01
影响因子：
4.2
通讯作者：
Forster, Robert
中科院分区：
文献类型：
--
作者：
Kong, Yunhong;Teather, Ronald;Forster, Robert
利用16 S rRNA基因克隆文库分析技术，研究了饲喂苜蓿和小黑麦的奶牛瘤胃细菌群落的种类组成、分布和多样性。将瘤胃细菌群落分成三个独立的部分进行分析：在瘤胃中的种群，松散地附着于瘤胃细菌颗粒，和紧密地附着于瘤胃细菌颗粒。从1014个测序克隆中鉴定出细菌9门19科32属613个操作分类单位（OTU）。613个OTU中有451个被确定为新种。这些细菌序列在饲喂苜蓿或小黑麦的奶牛瘤胃中的三个组分中分布不同。Chao 1估计表明，在两个群落中，与颗粒物紧密附着的种群比与颗粒物松散附着的种群更多样。S-Libshuff检测到任何两个部分之间的组成有显着差异，在瘤胃的奶牛与相同的饮食和社区之间的苜蓿和小黑麦饮食。苜蓿和小黑麦的群落物种丰富度估计分别为1027和662。在这项研究中检查的瘤胃细菌群落的多样性是大于以前的研究已经证明，两个高纤维饮食之间的社区组成的差异有影响下游宏基因组学应用的样品选择。
The species composition, distribution, and biodiversity of the bacterial communities in the rumen of cows fed alfalfa or triticale were investigated using 16S rRNA gene clone library analyses. The rumen bacterial community was fractionated and analyzed as three separate fractions: populations in the planktonic, loosely attached to rumen digesta particles, and tightly attached to rumen digesta particles. Six hundred and thirteen operational taxonomic units (OTUs) belonging to 32 genera, 19 families, and nine phyla of the domain Bacteria were identified from 1014 sequenced clones. Four hundred and fifty one of the 613 OTUs were identified as new species. These bacterial sequences were distributed differently among the three fractions in the rumen digesta of cows fed alfalfa or triticale. Chao 1 estimation revealed that, in both communities, the populations tightly attached to particulates were more diverse than the planktonic and those loosely attached to particulates. S-Libshuff detected significant differences in the composition between any two fractions in the rumen of cows with the same diet and between the communities fed alfalfa and triticale diets. The species richness estimated for the communities fed alfalfa and triticale is 1027 and 662, respectively. The diversity of the rumen bacterial community examined in this study is greater than previous studies have demonstrated and the differences in the community composition between two high-fiber diets have implications for sample selection for downstream metagenomics applications.