Delivery mode shapes the acquisition and structure of the initial microbiota across multiple body habitats in newborns
Delivery mode shapes the acquisition and structure of the initial microbiota across multiple body habitats in newborns
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DOI：
10.1073/pnas.1002601107
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发表时间：
2010-06-29
影响因子：
11.1
通讯作者：
Knight, Rob
中科院分区：
文献类型：
--
作者：
Dominguez-Bello, Maria G.;Costello, Elizabeth K.;Knight, Rob
在分娩时，新生儿第一次暴露于来自各种来源的各种微生物，包括母体细菌。尽管先前的研究表明，分娩模式塑造了微生物群的建立，随后又塑造了其在儿童健康中的作用，但大多数研究人员都专注于特定的细菌分类群或单一的身体栖息地，即肠道。因此，人类微生物组发育的起始阶段仍然不清楚。本研究的目的是获得一个社区范围内的角度来看，分娩方式和身体栖息地对新生儿的第一个微生物群的影响。我们使用多重16S rRNA基因焦磷酸测序来表征来自母亲及其新生儿的细菌群落，其中四个是阴道出生的，六个是通过剖腹产出生的。在分娩前1h采集母亲的皮肤、口腔粘膜和阴道样本，并采集新生儿的皮肤、口腔粘膜和鼻咽抽吸物样本
Upon delivery, the neonate is exposed for the first time to a wide array of microbes from a variety of sources, including maternal bacteria. Although prior studies have suggested that delivery mode shapes the microbiota's establishment and, subsequently, its role in child health, most researchers have focused on specific bacterial taxa or on a single body habitat, the gut. Thus, the initiation stage of human microbiome development remains obscure. The goal of the present study was to obtain a community-wide perspective on the influence of delivery mode and body habitat on the neonate's first microbiota. We used multiplexed 16S rRNA gene pyrosequencing to characterize bacterial communities from mothers and their newborn babies, four born vaginally and six born via Cesarean section. Mothers' skin, oral mucosa, and vagina were sampled 1 h before delivery, and neonates' skin, oral mucosa, and nasopharyngeal aspirate were sampled