Substoichiometric hydroxynonenylation of a single protein recapitulates whole-cell-stimulated antioxidant response.
Substoichiometric hydroxynonenylation of a single protein recapitulates whole-cell-stimulated antioxidant response.
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DOI：
10.1021/ja5084249
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发表时间：
2015-01-14
影响因子：
15
通讯作者：
Aye, Yimon
中科院分区：
文献类型：
--
作者：
Parvez, Saba;Fu, Yuan;Li, Jiayang;Long, Marcus J. C.;Lin, Hong-Yu;Lee, Dustin K.;Hu, Gene S.;Aye, Yimon
Lipid-derived electrophiles (LDEs) that can directly modify proteins have emerged as important small-molecule cues in cellular decision-making. However, because these diffusible LDEs can modify many targets [e.g., >700 cysteines are modified by the well-known LDE 4-hydroxynonenal (HNE)], establishing the functional consequences of LDE modification on individual targets remains devilishly difficult. Whether LDE modifications on a single protein are biologically sufficient to activate discrete redox signaling response downstream also remains untested. Herein, using T-REX (targetable reactive electrophiles and oxidants), an approach aimed at selectively flipping a single redox switch in cells at a precise time, we show that a modest level (∼34%) of HNEylation on a single target is sufficient to elicit the pharmaceutically important antioxidant response element (ARE) activation, and the resultant strength of ARE induction recapitulates that observed from whole-cell electrophilic perturbation. These data provide the first evidence that single-target LDE modifications are important individual events in mammalian physiology.
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DOI：
10.1074/mcp.m800070-mcp200
发表时间：
2009-04
期刊：
Molecular & cellular proteomics : MCP
影响因子：
--
作者：
Codreanu SG;Zhang B;Sobecki SM;Billheimer DD;Liebler DC
通讯作者：
Liebler DC
影响因子：
15
作者：
Fang X;Fu Y;Long MJ;Haegele JA;Ge EJ;Parvez S;Aye Y
通讯作者：
Aye Y
DOI：
10.1073/pnas.1305687110
发表时间：
2013-09-17
影响因子：
11.1
作者：
Baird, Liam;Lleres, David;Dinkova-Kostova, Albena T.
通讯作者：
Dinkova-Kostova, Albena T.
影响因子：
3.6
作者：
He, Xiaoqing;Ma, Qiang
通讯作者：
Ma, Qiang
影响因子：
7.4
作者：
Fritz, Kristofer S.;Petersen, Dennis. R.
通讯作者：
Petersen, Dennis. R.