Generation of an inducible, cardiomyocyte-specific transgenic mouse model with PPAR β/δ overexpression.
Generation of an inducible, cardiomyocyte-specific transgenic mouse model with PPAR β/δ overexpression.
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DOI：
10.1007/978-1-62703-155-4_4
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Yang, Qinglin
中科院分区：
文献类型：
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作者：
Kim, Teayoun;Zhelyabovska, Olga;Liu, Jian;Yang, Qinglin
过氧化物酶体增殖物激活受体（PPARs）由三种亚型组成，每种亚型显示出独特的组织分布。一般来说，三种PPAR亚型在脂质代谢的转录调控中发挥重叠的功能。然而，每种PPAR亚型在不同组织中具有不同的功能，这取决于它们在特定组织中的表达丰度、内源性配体和PPAR共调节因子。转基因是确定特定基因及其蛋白质的体内功能的宝贵技术。Cre/LoxP介导的基因打靶技术已被广泛用于研究PPARs的组织特异性功能。虽然这种组织特异性功能丧失方法在确定PPAR的基本作用方面非常有用，但组织特异性功能获得方法是用于了解特定组织中PPAR激活作用的另一种重要技术。已经使用了在特定组织中转基因过表达PPAR。然而，这种常规技术需要为每个靶组织单独生成转基因模型。在本章中，我们描述了一种更有效地产生转基因小鼠模型的方法，该模型在不同组织中具有组成型活性形式的PPARβ/δ。
Peroxisome proliferator-activated receptors (PPARs) consist of three subtypes, each displaying distinctive tissue distribution. In general, the three PPAR subtypes exert overlapping function in transcriptional regulation of lipid metabolism. However, each PPAR subtype possesses distinctive functions in different tissues dependent on their expression abundance, endogenous ligands, and the PPAR coregulators in a specific tissue. Transgenesis is an invaluable technique in defining the in vivo function of a particular gene and its protein. Cre/LoxP-mediated gene targeting has been extensively used to explore the tissue-specific function of PPARs. While this tissue-specific loss-of-function approach is extremely useful in determining the essential role of a PPAR, the tissue-specific gain-of-function approach is another important technique used to understand the effects of PPAR activation in a particular tissue. Transgenic overexpression of PPAR in a specific tissue has been used. However, this conventional technique requires generating the transgenic models individually for each target tissue. In this chapter, we describe the methodology for a more efficient generation of transgenic mouse models with a constitutively active form of PPARβ/δ in different tissues.