Lipopolysaccharide-binding protein of Bombyx mori participates in a hemocyte-mediated defense reaction against gram-negative bacteria
Lipopolysaccharide-binding protein of Bombyx mori participates in a hemocyte-mediated defense reaction against gram-negative bacteria
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DOI：
10.1016/s0022-1910(99)00069-4
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发表时间：
1999-09-01
影响因子：
2.2
通讯作者：
Sato, R
中科院分区：
文献类型：
--
作者：
Koizumi, N;Imai, Y;Sato, R
BmLBP是B中的脂多糖结合蛋白。mori并参与体内细菌清除。在这里，我们更具体地研究了BmLBP的功能。注射后30分钟内，超过90%的BmLBP结合的注射革兰氏阴性粗糙菌株从血浆中清除，而BmLBP不结合的光滑菌株需要8小时才能清除。对注射大肠杆菌粗糙株后血腔的观察表明，注射后30 min，当注射的大肠杆菌清除率下降时，血腔中形成黑色素结节。大肠杆菌细胞已经出现。荧光显微镜观察表明，E.大肠杆菌细胞实际上被困在体内形成的结节中。此外，等离子体预处理的E.将大肠杆菌粗糙细胞（BmLBP结合）加入到体外分离的血细胞中，引起血细胞与细菌的剧烈聚集，而血浆预处理的光滑细胞则没有。通过抗BmLBP血清预处理抑制聚集体的形成，表明BmLBP通过促进血细胞结节形成而引起细菌的清除。(C)1999爱思唯尔科技有限公司。保留所有权利。
BmLBP is a lipopolysaccharide-binding protein in B. mori and participates in bacterial clearance in vivo. Here, we investigated the function of BmLBP more specifically. More than 90% of injected gram-negative rough strains to which BmLBP binds were removed from the plasma within 30 min post-injection, whereas it required 8h for the clearance of smooth strains to which BmLBP does not bind. Observation of the hemocoel after the injection of Escherichia coli rough strain showed that melanized nodules were formed at 30 min post-injection when the clearance of injected E. coli cells had occurred. Fluorescence microscope observation revealed that E. coli cells were actually trapped in the nodules formed in vivo. Furthermore, plasma pre-treated E. coli rough cells (BmLBP bound) added to hemocytes isolated in vitro caused vigorous hemocyte aggregations with the bacteria, while plasma pretreated smooth cells did not. The formation of aggregates was inhibited by anti BmLBP serum pre-treatment, suggesting that BmLBP causes the clearance of bacteria by promoting hemocyte nodule formation. (C) 1999 Elsevier Science Ltd. All rights reserved.