Citronellal, a monoterpene present in Java citronella oil, attenuates mechanical nociception response in mice
Citronellal, a monoterpene present in Java citronella oil, attenuates mechanical nociception response in mice
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DOI：
10.3109/13880209.2013.781656
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发表时间：
2013-09-01
影响因子：
3.8
通讯作者：
Quintans-Junior, Lucindo Jose
中科院分区：
文献类型：
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作者：
de Santana, Marilia Trindade;Brito de Oliveira, Makson Gleydson;Quintans-Junior, Lucindo Jose
目的：研究香茅醛对不同刺激诱导的炎性伤害性反应的影响，探讨香茅醛对NO-cGMP-ATP敏感性K+通道通路的影响。材料和方法：选用雄性瑞士小鼠(每组6只)，在足底注射香茅素(25，50,100 mg/kg)0.5h后，足底注射L(CG；300mU g/paw)、肿瘤坏死因子-α(肿瘤坏死因子-α；100pg/paw)、前列腺素E-2(PGE(2)；100 ng/paw)或多巴胺(DA；30ug/paw)。分别于注药后0.5、1、2、3h用数字镇痛仪(Von Frey)评估痛觉。结果：所有剂量的香茅醛均能抑制CG(p<；0.001和p<；0.01)和肿瘤坏死因子-α(p<；0.001，p<；0.01，p<；0.05)诱导的机械伤害性反应。香茅醛在DA试验(p<；0.001，p<；0.01和p<；0.05)和前列腺素E(2)试验中(p<；0.001和p<；0.05)均能提高痛阈值。在PGE(2)实验中，L-NAME和格列本脲逆转了高剂量香茅醛的抗伤害性作用。讨论与结论：这些数据表明香茅醛减弱机械伤害性感觉，部分是通过NO-cGMP-ATP敏感性K+通道途径实现的。
Context: Citronellal is a monoterpene present in the oil of many species, including Cymbopogon winterianus Jowitt (Poaceae).Objective: The present study investigated the effect of citronellal on inflammatory nociception induced by different stimuli and examined the involvement of the NO-cGMP-ATP-sensitive K+ channel pathway.Materials and methods: We used male Swiss mice (n = 6 per group) that were treated intraperitoneally with citronellal (25, 50 or 100 mg/kg) 0.5 h after the subplantar injection of 20 mu l of carrageenan (CG; 300 mu g/paw), tumor necrosis factor-alpha (TNF-alpha; 100 pg/paw), prostaglandin E-2 (PGE(2); 100 ng/paw) or dopamine (DA; 30 mu g/paw). The mechanical nociception was evaluated at 0.5, 1, 2 and 3 h after the injection of the agents, using a digital analgesimeter (von Frey). The effects of citronellal were also evaluated in the presence of L-NAME (30 mg/kg) or glibenclamide (5 mg/kg).Results: At all times, citronellal in all doses inhibited the development of mechanical nociception induced by CG (p < 0.001 and p < 0.01) and TNF-alpha (p < 0.001, p < 0.01, and p < 0.05). The citronellal was able to increase the pain threshold in the DA test (p < 0.001, p < 0.01, and p < 0.05) and in the PGE(2) test at all times (p < 0.001 and p < 0.05). L-NAME and glibenclamide reversed the antinociceptive effects of the citronellal at higher doses in the PGE(2) test.Discussion and conclusion: These data suggest that citronellal attenuated mechanical nociception, mediated in part by the NO-cGMP-ATP-sensitive K+ channel pathway.