ET3/Ednrb2 Signaling Is Critically Involved in Regulating Melanophore Migration in Xenopus
ET3/Ednrb2 Signaling Is Critically Involved in Regulating Melanophore Migration in Xenopus
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DOI：
10.1002/dvdy.22649
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发表时间：
2011-06-01
影响因子：
2.5
通讯作者：
Yamamoto, Hiroaki
中科院分区：
文献类型：
--
作者：
Kawasaki-Nishihara, Akiha;Nishihara, Daisuke;Yamamoto, Hiroaki
成黑素细胞来源于神经嵴细胞（NCC），是唯一通过背外侧通路迁移的NCC。然而，成黑素细胞如何通过不同于其他NCC的途径进化迁移仍然不清楚，因为对物种之间保守的成黑素细胞迁移的共同分子机制知之甚少。内皮素受体B2（Ednrb 2）是鸟类成黑素细胞进入背外侧通路所必需的。在这里，我们表明，内皮素-3（ET 3）/Ednrb 2信号也需要黑素细胞迁移非洲爪蟾，虽然他们通过腹侧途径迁移。在非洲爪蟾中，Ednrb 2由迁移前阶段的成黑素细胞表达，而ET 3在其目的地周围表达，这表明ET 3/Ednrb 2信号传导可能决定黑素细胞定位。此外，黑素细胞迁移在体内被异常的ET 3/Ednrb 2信号传导中断，并且它们的侵袭能力在体外被ET 3增强。我们的研究结果表明，ET 3/Ednrb 2信号是必需的黑素细胞迁移Ednrb 2基因保守的动物。Developmental Dynamics 240：1454-1466，2011. (C)2011 Wiley-Liss，Inc.
Melanoblasts are derived from neural crest cells (NCCs) and are the only NCCs that migrate through the dorsolateral pathway. However, how melanoblasts evolved to migrate through a pathway different from other NCCs is still unclear, because little is known about common molecular mechanisms of melanoblast migration that are conserved between species. Endothelin receptor B2 (Ednrb2) is required for avian melanoblasts to enter the dorsolateral pathway. Here, we show that Endothelin-3 (ET3)/Ednrb2 signaling is also required for melanoblast migration in Xenopus laevis, although they migrate through the ventral pathway. In Xenopus, Ednrb2 is expressed by melanoblasts from pre-migration stages and ET3 is expressed around their destinations, suggesting that ET3/Ednrb2 signaling may determine melanophore localization. Furthermore, melanoblast migration is interrupted by aberrant ET3/Ednrb2 signaling in vivo and their invasive ability is enhanced by ET3 in vitro. Our results suggest that ET3/Ednrb2 signaling is required for melanoblast migration in Ednrb2 gene-conserved animals. Developmental Dynamics 240:1454-1466, 2011. (C) 2011 Wiley-Liss, Inc.