Progress toward ultrafast DNA Sequencing using solid-state nanopores
Progress toward ultrafast DNA Sequencing using solid-state nanopores
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DOI：
10.1373/clinchem.2007.091231
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发表时间：
2007-11-01
影响因子：
9.3
通讯作者：
Meller, Amit
中科院分区：
文献类型：
--
作者：
Soni, Gautam V.;Meller, Amit
背景资料：对流经纳米级孔（纳米孔）的离子电流的测量已被用于分析单个DNA和RNA分子，最终目标是实现超快DNA测序。然而，纯电子测量的尝试没有实现单核苷酸区分所需的信号对比度。在这份报告中，我们提出了一种新的方法的光学检测的DNA序列易位通过nanopore.Methods：每个碱基的目标DNA序列是第一次映射到一个2单位的代码，2个10-bp的核苷酸序列，通过生化转化成设计的DNA聚合物。然后将这些2单元代码与互补的、荧光标记的和自猝灭的分子信标杂交。当分子信标在通过一个
Background: Measurements of the ionic current flowing through nanometer-scale pores (nanopores) have been used to analyze single DNA and RNA molecules, with the ultimate goal of achieving ultrafast DNA sequencing. However, attempts at purely electronic measurements have not achieved the signal contrast required for single nucleotide differentiation. In this report we propose a novel method of optical detection of DNA sequence translocating through a nanopore.Methods: Each base of the target DNA sequence is 1st mapped onto a 2-unit code, 2 10-bp nucleotide sequence, by biochemical conversion into Designed DNA Polymers. These 2-unit codes are then hybridized to complementary, fluorescently labeled, and self-quenching molecular beacons. As the molecular beacons are sequentially unzipped during translocation through a