Global gene expression of rhizobacteria-silicon mediated induced systemic resistance in tomato (Solanum lycopersicum) against Ralstonia solanacearum
Global gene expression of rhizobacteria-silicon mediated induced systemic resistance in tomato (Solanum lycopersicum) against Ralstonia solanacearum
复制标题
DOI：
10.1016/j.pmpp.2013.06.004
复制
发表时间：
2013-10-01
影响因子：
2.7
通讯作者：
Wydra, Kerstin
中科院分区：
文献类型：
--
作者：
Kurabachew, Henok;Stahl, Frank;Wydra, Kerstin
对番茄 (Solanum lycopersicum) 茎组织进行转录组分析，以阐明在接种青枯雷尔斯顿菌菌株 ToUdk2（种族 1，源自泰国的系统发育型 1）后，用硅和/或根际细菌菌株短小芽孢杆菌处理的植物中的基因表达分析。共有174个基因被差异调节，其中113个上调，61个下调。对调节基因的功能分类显示，大多数上调基因更多地参与信号转导、防御、蛋白质合成和代谢，而很大一部分下调基因更多地参与光合作用、脂质代谢。在用硅处理的植物中，与用短小芽孢杆菌处理的植物相比，更多数量的防御相关基因受到调节。在同时应用两个诱导子期间，五个基因下调，而单独应用时五个基因上调，表明乙烯-茉莉酸和水杨酸途径介导的基因之间发生拮抗相互作用。因此，建议单独应用每种诱导子作为诱导番茄青枯病抗性的最佳策略，其中硅是比根际细菌更强的诱导剂。 (C) 2013 Elsevier Ltd. 保留所有权利。
Transcriptome analysis of tomato (Solanum lycopersicum) stem tissue was performed to elucidate gene expression analysis in plant treated with silicon and/or rhizobacteria strain Bacillus pumilis following inoculation with Ralstonia solanacearum strain ToUdk2 (race 1,phylotype 1 originated from Thailand). A total of 174 genes were differentially regulated of which 113 were up-regulated and 61 down-regulated functional categorization of the regulated genes revealed that most of the up-regulated genes are more involved in signal transduction, defence, protein synthesis and metabolism, while a large proportion of down regulated genes were more involved in photosynthesis, lipid metabolism. In plant treated with silicon a higher number of defence related genes were regulated than those treated with rhizobacterium B. pumilis. During the simultaneous application of the two elicitors five genes were down regulated, while they were up-regulated when they were applied separately, suggesting that an antagonistic interaction occurred between genes mediated by ethylene-jasmonate and salicylate pathways. Therefore, separate application of each elicitor is recommended as best strategy for the induction of resistance against bacterial wilt in tomato, with silicon being a stronger inducer than rhizobacteria. (C) 2013 Elsevier Ltd. All rights reserved.