High-performance liquid chromatographic determination of propranolol and 4-hydroxypropranolol in plasma.
High-performance liquid chromatographic determination of propranolol and 4-hydroxypropranolol in plasma.
复制标题
高效液相色谱法测定血浆中普萘洛尔和4-羟基普萘洛尔。
DOI：
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发表时间：
1981
期刊：
影响因子：
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通讯作者：
M. Bogaert
中科院分区：
文献类型：
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作者：
M. Rosseel;M. Bogaert
建立了高效液相色谱法同时测定血浆中普萘洛尔和4-羟基普萘洛尔的含量。该方法包括在碱性pH下用乙基柱进行血浆萃取，并进行荧光检测。普萘洛尔的反相柱变异系数为3.0 ~ 7.1%，4-羟基普萘洛尔的反相柱变异系数为5.8 ~ 8.3%。普萘洛尔和4-羟基普萘洛尔的日变化分别为4.6%和8.2%。该方法可检测每种化合物1 ng /ml的血浆。
A high-performance liquid chromatographic method for the simultaneous determination of propranolol and 4-hydroxypropranolol in plasma is presented. The method involves plasma extraction at basic pH with ethyl column, and fluorescence detection. The within-run a reversed-phase column coefficient of variation were 3.0-7.1% for propranolol and 5.8-8.3% for 4-hydroxypropranolol. The day-to-day variations were 4.6 and 8.2% for propranolol and 4-hydroxypropranolol, respectively. The method can detect 1 ng of each compound/ml of plasma.