Three different anti-lipopolysaccharide factors identified from giant freshwater prawn, Macrobrachium rosenbergii
Three different anti-lipopolysaccharide factors identified from giant freshwater prawn, Macrobrachium rosenbergii
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从罗氏沼虾中鉴定出三种不同的抗脂多糖因子
DOI：
10.1016/j.fsi.2012.06.032
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发表时间：
2012-10-01
影响因子：
4.7
通讯作者：
Wang, Wen
中科院分区：
文献类型：
--
作者：
Ren, Qian;Zhang, Zhao;Wang, Wen
抗脂多糖因子(ALF)是一种碱性蛋白，是一种重要的结合和中和脂多糖(LPS)的抗菌肽。这种蛋白质显示出广谱的抗菌活性。在本研究中，从罗氏沼虾中鉴定了三种ALF，分别命名为Mr ALF5、Mr ALF6和Mr ALF7。MrALF5、mrALF6和mrALF7基因分别编码133、121和120个氨基酸。所有这些ALF蛋白都含有内毒素结合区和两个保守的半胱氨酸残基。扩增出了MRALF5和MRALF7的基因组序列。MRALF5和MRALF7的基因组结构由三个外显子和两个内含子组成。系统发育分析表明，MRALF5、MRALF6和MRALF7聚为分支II。进化分析表明，罗氏沼虾ALF基因可能经历了快速进化。MRALF5主要在肝胰腺、鳃和心脏中表达。MRALF6主要分布在肠道和肝胰腺。MRALF7在肝胰腺中的表达水平最高。MRALF6和MRALF7被大肠杆菌攻击下调，三个ALF基因都被弧菌或白斑综合征病毒攻击上调。金黄色葡萄球菌也上调了MRALF6的表达。综上所述，ALF基因的三种异构体可能参与了对虾感染细菌和病毒的先天免疫反应。(C)2012爱思唯尔有限公司。保留所有权利。
Anti-lipopolysaccharide factor (ALF) is a type of basic protein and an important antimicrobial peptide that can bind and neutralize lipopolysaccharides (LPS). This protein shows a broad spectrum of antimicrobial activity. In this study, three forms of ALF designated as MrALF5, MrALF6, and MrALF7 were identified from giant freshwater prawn, Macrobrachium rosenbergii. MrALF5, MrALF6, and MrALF7 genes encode 133, 121, and 120 amino acids of the corresponding proteins, respectively. All these ALF proteins contain LPS-binding domain with two conserved cysteine residues. The genomic sequences of MrALF5 and MrALF7 were amplified. The genomic structures of MrALF5 and MrALF7 comprise three exons interrupted by two introns. Phylogenetic analysis showed that MrALF5, MrALF6, and MrALF7 were clustered into clade II. Evolutionary analysis showed that ALF genes from M. rosenbergii may suffer a rapid evolution. MrALF5 was expressed mainly in the hepatopancreas, gills, and heart. MrALF6 was mainly distributed in the intestine and hepatopancreas. The highest expression level of MrALF7 was detected in the hepatopancreas. MrALF6, as well as MrALF7, was downregulated by Escherichia coli challenge, and all three ALF genes were upregulated by Vibrio or white spot syndrome virus challenge. MrALF6 was also upregulated by Staphylococcus aureus challenge. In summary, the three isoforms of ALF genes may participate in the innate immune response against bacteria and virus infecting the giant fresh water prawn. (C) 2012 Elsevier Ltd. All rights reserved.