Cell transport via electromigration in polymer-based microfluidic devices
Cell transport via electromigration in polymer-based microfluidic devices
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DOI：
10.1039/b317093d
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发表时间：
2004-01-01
期刊：
影响因子：
6.1
通讯作者：
Soper, SA
中科院分区：
文献类型：
--
作者：
Witek, MA;Wei, SY;Soper, SA
用微流控装置对大肠杆菌和酿酒酵母细胞的电动力学传输进行了研究，微流控装置分别由原始和紫外线修饰的聚甲基丙烯酸甲酯（PMMA）和聚碳酸酯（PC）制成。芯片间细胞表观迁移率（mu(app)）的重现性略有不同，RSD近似于10%。在0.5 mM PBS （pH = 7.4）的uv改性PC中，面包酵母细胞的mapp最高，在20 mM PBS （pH = 7.4）的原始PMMA中，mapp最低。所有装置中的贝克酵母都向阴极迁移，因为它们的电泳迁移率比EOF小。在0.5 mM和1mm PBS中，大肠杆菌细胞在所有情况下都向阳极迁移，与EOF的方向相反，因为它们的电泳迁移率更大。大肠杆菌细胞在20 mM PBS中向阴极迁移，表明在较高的离子强度下，大肠杆菌细胞的电泳迁移率降低。以观察到的大肠杆菌和面包酵母细胞在适当制备的聚合物微芯片上的差异迁移为基础，选择性地将一种细胞引入微流控装置。作为工作模型，用大肠杆菌和红细胞进行实验。红细胞在含有1 mM和20 mM PBS （pH = 7.4）的原始PMMA中向阴极迁移，与大肠杆菌细胞的方向相反。通过合理选择制备细胞悬浮液和聚合物材料的缓冲液浓度，将红细胞或大肠杆菌细胞选择性导入微装置，通过激光后向散射信号监测微装置的状态。
Electrokinetic transport of Escherichia coli and Saccharomyces cerevisiae (baker's yeast) cells was evaluated in microfluidic devices fabricated in pristine and UV-modified poly(methyl methacrylate) (PMMA) and polycarbonate ( PC). Chip-to-chip reproducibility of the cell's apparent mobilities (mu(app)) varied slightly with a RSD of similar to10%. The highest mapp for baker's yeast cells was observed in UV-modified PC with 0.5 mM PBS (pH = 7.4), and the lowest was measured in pristine PMMA with 20 mM PBS (pH = 7.4). Baker's yeast in all devices migrated toward the cathode because of their smaller electrophoretic mobility compared to the EOF. In 0.5 mM and 1 mM PBS, E. coli cells migrated toward the anode in all cases, opposite to the direction of the EOF due to their larger electrophoretic mobility. E. coli cells in 20 mM PBS migrated toward the cathode, which indicated that the electrophoretic mobility of E. coli cells decreased at higher ionic strengths. Observed differential migrations of E. coli and baker's yeast cells in appropriately prepared polymer microchips were used as the basis for selective introduction into microfluidic devices of only one type of cell. As a working model, experiments were performed with E. coli and RBCs (red blood cells). RBCs migrated toward the cathode in pristine PMMA with 1 mM and 20 mM PBS (pH = 7.4), opposite to the direction of the E. coli cells. By judicious choice of the buffer concentration in which the cell suspension was prepared and the polymer material, RBCs or E. coli cells were selectively introduced into the microdevice, which was monitored via laser backscatter signals.