MAST-CELLS IN ULCERATIVE-COLITIS - QUANTITATIVE AND ULTRASTRUCTURAL STUDIES
MAST-CELLS IN ULCERATIVE-COLITIS - QUANTITATIVE AND ULTRASTRUCTURAL STUDIES
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DOI：
10.1007/bf02899101
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发表时间：
1989-01-01
期刊：
影响因子：
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通讯作者：
VADASZ, G
中科院分区：
文献类型：
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作者：
BALAZS, M;ILLYES, G;VADASZ, G
研究人员对 37 例溃疡性结肠炎患者的结肠镜活检中肥大细胞数量和超微结构的变化进行了研究。比较疾病活动期和缓解期的变化。在四氧化锇固定后用吉姆萨染色的半薄切片上进行细胞计数。该方法克服了福尔马林固定后发现的不确定染色。发现肥大细胞的积累伴随着强烈的脱颗粒在疾病的活动期是显着的。观察到两种形式的脱颗粒：单个颗粒的排出以及含有颗粒的细胞质突起的突出和脱离。正如前面在动物实验中所描述的，后者是快速脱粒的标志。肥大细胞与毛细血管、雪旺细胞、神经纤维、肌成纤维细胞和胶原纤维密切相关，也存在于上皮细胞之间。假设紧密的地形接触也可能意味着功能相关性。
The changes in the number and ultrastructure of mast cells were studied in 37 colonoscopical biopsies from patients with ulcerative colitis. Changes in the active stage of the disease and during remission were compared. Cell counts were performed on semithin sections stained with Giemsa after osmium tetroxide fixation. This method overcome the uncertain staining found after formalin fixation. Accumulation of mast cells accompanied by intense degranulation was found to be significant in the active stage of the disease. Two forms of degranulation were observed: discharge of the individual granules and protrusion and detachment of the cytoplasmic processes containing granules. The latter was a sign of rapid degranulation, as described earlier in animal experiments. Mast cells were closely associated with capillary blood vessels, Schwann cells, neural fibres, myofibroblasts and collagenous fibres, and were also present between epithelial cells. It is assumed that close topographic contact may also imply a functional correlation.