Oral crocetin administration suppressed refractive shift and axial elongation in a murine model of lens-induced myopia
Oral crocetin administration suppressed refractive shift and axial elongation in a murine model of lens-induced myopia
复制标题
DOI：
10.1038/s41598-018-36576-w
复制
发表时间：
2019-01-22
影响因子：
4.6
通讯作者：
Tsubota, Kazuo
中科院分区：
文献类型：
--
作者：
Mori, Kiwako;Kurihara, Toshihide;Tsubota, Kazuo
全球近视发病率的增加需要建立针对其进展的治疗方法。为了探索可以控制近视的药物，我们基于近视抑制因子早期生长反应蛋白1（Egr-1）的体外活性筛选了207种天然化合物和化学试剂。在候选者中，西红花酸显示出最高的Egr-1的剂量依赖性激活。对于体内分析，在3周龄C57 BL/6 J小鼠中用~ 30屈光度（D）镜片诱导实验性近视3周。在近视诱导期间，动物喂食含有0.003%（n = 19）和0.03%（n = 7）西红花酸的正常或混合食物。在3周龄和6周龄时用红外线验光仪和SD-OCT系统测量屈光和眼轴长度。与对照组（n = 14）相比，西红花酸给药组的屈光不正变化显著较小（0.003%为-13.62 +/- 8.14 vs + 0.82 +/- 5.81 D，p < 0.01，0.03%为-2.00 +/- 4.52 D，p < 0.01）和轴向伸长（0.003%为0.27 +/- 0.03对0.22 +/- 0.04 mm，p < 0.01，0.03%为0.23 +/- 0.05 mm，p < 0.05）。这些结果表明，膳食因素藏红花酸可能对近视进展有预防作用。
Increased global incidence of myopia necessitates establishment of therapeutic approaches against its progression. To explore agents which may control myopia, we screened 207 types of natural compounds and chemical reagents based on an activity of a myopia suppressive factor, early growth response protein 1 (Egr-1) in vitro. Among the candidates, crocetin showed the highest and dose-dependent activation of Egr-1. For in vivo analysis, experimental myopia was induced in 3-week-old C57BL/6 J mice with -30 diopter (D) lenses for 3 weeks. Animals were fed with normal or mixed chow containing 0.003% (n = 19) and 0.03% (n = 7) of crocetin during myopia induction. Refraction and axial length were measured at 3-week-old and the 6-week-old with an infrared photorefractor and a SD-OCT system. Compared to controls (n = 14), crocetin administration showed a significant smaller change of refractive errors (-13.62 +/- 8.14 vs + 0.82 +/- 5.81 D for 0.003%, p < 0.01, -2.00 +/- 4.52 D for 0.03%, p < 0.01) and axial elongation (0.27 +/- 0.03 vs 0.22 +/- 0.04 mm for 0.003%, p < 0.01, 0.23 +/- 0.05 mm for 0.03%, p < 0.05). These results suggest that a dietary factor crocetin may have a preventive effect against myopia progression.