Current-modulated electrical stimulation as a treatment for peripheral nerve regeneration in diabetic rats
Current-modulated electrical stimulation as a treatment for peripheral nerve regeneration in diabetic rats
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DOI：
10.3233/rnn-130381
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发表时间：
2014-01-01
影响因子：
2.8
通讯作者：
Chen, Yueh-Sheng
中科院分区：
文献类型：
--
作者：
Lin, Yu-Ching;Kao, Chia-Hong;Chen, Yueh-Sheng
目的：研究电刺激（ES）是否可以成为一个有用的工具，以改善周围神经系统神经元损伤后的功能恢复方法：我们研究了2 Hz的经皮ES在不同强度的1，10和20 mA的链脲佐菌素诱导的糖尿病大鼠周围神经再生的影响。未受刺激的糖尿病大鼠用作假手术对照。结果：糖尿病大鼠恢复4周后，1 mA以上的ES可使损伤侧后肢皮肤血流量增加，而1 mA以上的ES可使损伤侧后肢皮肤血流量增加，并可使损伤侧后肢皮肤血流量增加，而1 mA以上的ES可使损伤侧后肢皮肤血流量增加。10 mA电刺激可提高靶肌诱发动作电位的幅值和面积，使靶肌神经再支配速度加快。10 mA的ES还可以改善损伤同侧背角I-II层区降钙素基因相关肽的表达和糖尿病远端坐骨神经中巨噬细胞的数量。结论：10 mA以上的电刺激对糖尿病大鼠坐骨神经损伤后再生有促进作用。
Purpose: To study if electrical stimulation (ES) can be a useful tool to improve functional recovery after neuronal injury in the peripheral nervous system.Methods: We studied the effects of 2 Hz of percutaneous ES at different intensities of 1, 10 and 20 mA on peripheral nerve regeneration in rats with diabetes induced by streptozotocin. Non-stimulated diabetic rats were used as the sham-controls. A10-mm gap was made in the rat sciatic nerve by suturing the stumps into silicone rubber tubes and stimulation was carried out every other day for 3 weeks starting 1 week after surgery.Results: After 4 weeks of recovery, the diabetic rats showed that ES of I mA or above could increase the cutaneous blood flow in their ipsilateral hindpaw to the injury. ES of 10 mA could improve the amplitude and the area of evoked muscle action potentials with faster target muscle reinnervation. ES of 10 mA could also ameliorate the calcitonin gene-related peptide expression in lamina I-II regions in the dorsal horn ipsilateral to the injury and the number of macrophages in the diabetic distal sciatic nerve. The impaired growth and maturation of regenerating axons in diabetic rat could be improved by ES of 10 mA or above.Conclusions: All these results lead to the conclusion that ES of 10 mA or above might be necessary to improve regeneration after a dissect lesion of the sciatic nerve in the diabetic rat.