Oxygenation of benzo[a]pyrene by plant microsomal fractions
Oxygenation of benzo[a]pyrene by plant microsomal fractions
复制标题
植物微粒体部分对苯并[a]芘的氧化
DOI：
10.1016/0014-5793(80)80258-4
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发表时间：
1980
期刊：
影响因子：
3.5
通讯作者：
H. Sandermann
中科院分区：
文献类型：
--
作者：
Th.v.d. Trenck;H. Sandermann
苯并[a]芘是一种普遍存在的高度致癌化合物[1，2]。据估计，仅在美国每年就释放894吨，主要是在燃烧过程中[11.BP在哺乳动物系统中广泛代谢，特别是通过肝脏微粒体的混合功能加氧酶系统[3]。细胞色素P450的种类参与了这一过程，并通过环氧化物中间体将其引导为各种酚类和二氢二酚类代谢物。一种明确的二醇环氧化物被认为是通过与细胞DNA共价结合而起到最终致癌作用的[3]。BP是一种相对稳定的化合物[1，2]，例如，在废物处理条件下是完全惰性的[4]，但已发现它被某些微生物[5]、完整的植物[6、7]和培养的植物细胞[8、9]代谢。导致BP不同植物代谢物组分的生化反应尚不清楚，尽管根据高效液相数据[LO]推测了与肝微粒体系统的某些相似之处。目前报道，植物微体部分催化BP的氧化，这一过程与肝脏微体部分催化BP的氧化有很大的不同。
Benzo [a] pyrene is a highly carcinogenic compound that occurs universally [1, 2]. An estimated 894 tons are released annually in the US alone, mainly in combustion processes [11.BP is extensively metabolized in mammalian systems, in particular by the mixed-function oxygenase system of liver microsomes [3]. Species of cytochrome P450 are involved in this process and lead via epoxide intermediates to various phenolic and dihydrodiol metabolites. A defined diol-epoxide is though to act as the ultimate carcinogen by binding covalently to cellular DNA [3]. BP is a relatively stable compound [1, 2] which is, for example, completely inert under waste treatment conditions [4] but it has been found to be metabolized by certain microorganisms [5], by intact plants [6, 7] and by cultured plant cells [8, 9]. The biochemical reactions leading to the various plant metabolite fractions of BP are as yet unknown although certain analogies with the liver microsomal system have been postulated on the basis of HPLC data [lo]. It is now reported that plant microsomal fractions catalyze the oxygenation of BP and that this process differs considerably from the oxygenation of BP by liver microsomal fractions.