The influence of addition of gallic acid, tannic acid, or quebracho tannins to alfalfa hay on in vitro rumen fermentation and microbial protein synthesis
The influence of addition of gallic acid, tannic acid, or quebracho tannins to alfalfa hay on in vitro rumen fermentation and microbial protein synthesis
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DOI：
10.1016/j.anifeedsci.2007.03.011
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发表时间：
2008-01-15
影响因子：
3.2
通讯作者：
DePeters, E. J.
中科院分区：
文献类型：
--
作者：
Getachew, G.;Pittroff, W.;DePeters, E. J.
单宁可以降低蛋白质的瘤胃降解率，增加饲料蛋白质到达下消化道进行酶消化的比例，从而提高蛋白质的利用效率。以紫花苜蓿（Medicago sativa L.）为试验材料，研究了不同类型和水平单宁对瘤胃体外产气量及其动力学、蛋白质体外真降解率（IVTD）和瘤胃降解率（IVRDP）以及微生物蛋白质合成的影响。干草在缓冲的瘤胃液中。在添加和不添加不同水平的没食子酸（GA）、白坚木单宁（QT）或单宁酸（TA）的情况下，在缓冲的瘤胃液中孵育Alcohol。与较高水平相比，较低包含水平的单宁对发酵产物的影响最小。添加QT和TA降低了铵态氮（NH_4 ~+-N）浓度。与对照相比，添加20、40和60 g/kg DM的QT使NH 4 +-N分别降低了2%、7%和12%，而添加20、40和60 g/kg DM的TA使NH 4 +-N分别降低了5%、6%和12%。在试验2中，添加QT在50，100，和150 g/kg DM，导致减少NH 4 +-N的12%，30%，和51%，分别与对照相比。与对照相比，添加50、100和150 g/kdDM的TA使NH 4 +-N降低了14、26和47%。与对照组相比，在50、100、150 DM时纳入QT使IVRDP降低13、30和36%，而在这些纳入水平下，TA使IVRDP降低14、25和48%。产气率下降（P
Tannins may reduce rumen degradability of protein, increase the proportion of feed protein reaching the lower digestive tract for enzymatic digestion and thereby increase the efficiency of protein utilization. The objective was to assess the effects of different types and levels of tannins on rumen in vitro gas production and its kinetics, in vitro true degradability (IVTD) and rumen degradability of protein (IVRDP), and microbial protein synthesis by incubating alfalfa (Medicago sativa L.) hay in buffered rumen fluid. Alfalfa was incubated in buffered rumen fluid with and without the addition of different levels of gallic acid (GA), quebracho tannin (QT), or tannic acid (TA). Tannins at the lower inclusion levels had minimal effects on fermentation products compared to the higher levels. Addition of QT and TA reduced ammonium-N (NH4+-N) concentration. Addition of QT at 20, 40, and 60 g/kg DM decreased NH4+-N by 2, 7, and 12% compared with control whereas addition of TA reduced NH4+-N by 5, 6, and 12% when added at 20, 40 and 60 g/kg DM, respectively. In experiment 2, addition of QT at 50, 100, and 150 g/kg DM, resulted in reduction of NH4+-N by 12, 30, and 51%, respectively, compared with the control. Addition of TA at 50, 100, and 150 g/kd DM reduced NH4+-N by 14, 26, and 47% compared with control. Inclusion of QT at 50, 100, 150 DM reduced IVRDP by 13, 30, and 36% compared with control whereas at these levels of inclusion, TA resulted in reduction of IVRDP by 14, 25, and 48%. Rate of gas production decreased (P