SPARSE SEEDING OF BONE-MARROW AND THYMUS IN RADIATION CHIMAERAS
SPARSE SEEDING OF BONE-MARROW AND THYMUS IN RADIATION CHIMAERAS
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DOI：
10.1097/00007890-197501000-00002
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发表时间：
1975-01-01
期刊：
影响因子：
6.2
通讯作者：
DAVIES, AJS
中科院分区：
文献类型：
--
作者：
WALLIS, VJ;LEUCHARS, E;DAVIES, AJS
研究了小鼠胸腺在潜在致死剂量照射和注射不同数量的同基因但染色体可区分的骨髓细胞后的再生情况。注射的骨髓细胞越多，从分裂的宿主细胞到分裂的供体细胞的转变就越早。在照射后30天和60天，外周血中对植物血凝素有反应的供体来源的T细胞数量与注射的供体骨髓细胞数量成正比，但宿主来源的T细胞数量成反比。在辐照后注射两种同基因染色体可区分的造血细胞群的混合物的实验表明，相对少量的造血细胞可以负责重新填充单个股骨。对胸腺的分析表明，胸腺内的分裂细胞群也可以来自很少的前体细胞，可能只有一个或两个。
The repopulation of the thymus was studied in mice after a potentially lethal dose of irradiation and injection of different numbers of syngeneic but chromosomally distinguishable bone marrow cells. The more bone marrow cells were injected, the earlier was the changeover from dividing host cells to dividing donor cells observed. At both 30 and 60 days after irradiation, the number of donor-derived T cells in the peripheral blood responding to phytohemagglutinin was directly proportional to the number of donor bone marrow cells injected, but the number of host-derived T cells was inversely proportional. Experiments in which mixtures of two syngeneic chromosomally distinguishable haematopoietic cell populations were injected after irradiation suggested that relatively small numbers of haematopoietic cells can be responsible for repopulating a single femur. Analysis of the thymus showed that the dividing cell populations within the thymus can also derive from very few precursor cells, possibly as few as one or two.