Organelle-Targeted H2S Probes Enable Visualization of the Subcellular Distribution of H2S Donors.
Organelle-Targeted H2S Probes Enable Visualization of the Subcellular Distribution of H2S Donors.
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DOI：
10.1021/acs.analchem.6b00087
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发表时间：
2016-06-07
影响因子：
7.4
通讯作者：
Pluth MD
中科院分区：
文献类型：
--
作者：
Montoya LA;Pluth MD
硫化氢（H2S）是多种生物调节途径中必不可少的生物信号分子。为了提供新的化学工具H2S成像，我们在这里报告的荧光H2S检测平台（HSN 2-BG），是兼容的亚细胞定位SNAP标签融合蛋白的方法，并使用适当的融合蛋白的结构，以证明线粒体和溶酶体的定位。我们还证明了该检测平台对中国仓鼠卵巢（CHO）细胞中内源性H2S进行成像的有效性，并使用开发的构建体报告了常见H2S供体分子AP 39、ADT-OH、GYY 4137和二烯丙基三硫化物（DATS）提供的亚细胞H2S分布。所开发的构建体提供了一个平台，准备提供新的见解的亚细胞分布的常见H2S供体和一个有用的工具，用于调查H2S生物化学。
Hydrogen sulfide (H2S) is an essential biological signaling molecule in diverse biological regulatory pathways. To provide new chemical tools for H2S imaging, we report here a fluorescent H2S detection platform (HSN2-BG) that is compatible with subcellular localization SNAP-tag fusion protein methodologies and use appropriate fusion protein constructs to demonstrate mitochondrial and lysosomal localization. We also demonstrate the eӽcacy of this detection platform to image endogenous H2S in Chinese hamster ovary (CHO) cells and use the developed constructs to report on the subcellular H2S distributions provided by common H2S donor molecules AP39, ADT–OH, GYY4137, and diallyltrisulfide (DATS). The developed constructs provide a platform poised to provide new insights into the subcellular distribution of common H2S donors and a useful tool for investigating H2S biochemistry.