Quantification of fluoxetine and norfluoxetine serum levels by reversed-phase high-performance liquid chromatography with ultraviolet detection
Quantification of fluoxetine and norfluoxetine serum levels by reversed-phase high-performance liquid chromatography with ultraviolet detection
复制标题
DOI：
10.1016/s0378-4347(97)00470-2
复制
发表时间：
1997-12-19
期刊：
影响因子：
--
通讯作者：
Yoo, SD
中科院分区：
文献类型：
--
作者：
Holladay, JW;Dewey, MJ;Yoo, SD
建立了一种快速、灵敏的高效液相色谱法测定血清中氟西汀和去甲氟西汀浓度。使用移动的流动相（55%乙腈-45%蒸馏水，含有10 mM三乙胺水溶液）通过在226 nm处的紫外检测来分离氟西汀和去甲氟西汀（25-1000 ng/ml，使用氯丙咪嗪作为内标）。氟西汀和去甲氟西汀的日间和日内变异系数均为13- 18%，回收率均大于89%。将该方法应用于氟西汀在小鼠体内的药代动力学研究。(C)1997年Elsevier Science B.V.
A rapid and sensitive high-performance liquid chromatography assay method was developed to determine serum fluoxetine and norfluoxetine levels by single extraction of 0.1 ml of serum with sodium hydroxide. The mobile phase (55% acetonitrile-45% distilled water containing 10 mM aqueous triethylamine) was used to separate fluoxetine and norfluoxetine (25-1000 ng/ml, using clomipramine as the internal standard) by ultraviolet detection at 226 nm. The inter- and intra-day variabilities of fluoxetine and norfluoxetine were 13-18%, and the recoveries of both drugs exceeded 89%. This assay method was applied to a pharmacokinetic disposition study of fluoxetine in mice. (C) 1997 Elsevier Science B.V.